• • DRAMP ID

  • DRAMP00361

• • Peptide Name

  • Non-specific lipid-transfer protein (LTP; Harmalin; Plants)

• • Source

  • Peganum harmala (Syrian rue) (Harmal peganum)

• • Family

  • Belongs to the plant LTP family

• • Gene

  • Not found

• • Sequence

  • ITCPQVTQSLAPCVPYLISG

• • Sequence Length

  • 20

• • UniProt Entry

  • B3EWH4

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antifungal, Antiviral, Anti-cancer

• • Target Organism

  • • Fungi: Penicillium digitatum (IC50=37.5 µM), Alternaria alternata (IC50=1.5 µM), Rhizopus stolonifer (IC50=8.44 µM) and Magnaporthe grisea (IC50=12.19 µM).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Lipid-binding

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP00361.

DRAMP00361

• • Function

  • Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues. Inhibits cell proliferation of cervical carcinoma cell line HeLa (IC50=2.74 µM), gastric carcinoma cell line MGC-7 (IC50=3.13 µM), esophageal carcinoma cell line Eca-109 (IC50=0.7 µM) and melanoma B16 cells (IC50=1.47 µM). Has antifungal activity. Induces caspase-dependent apoptosis in cell line Eca-109. Demonstrates inhibitory effect on HIV-1 reverse transcriptase (IC50=1.26 µM).

• • Biophysicochemical properties

  • pH dependence (Stable between pH 4 and 10); Temperature dependence (Thermostable. Retains antifungal activity between 4 degrees Celsius and 60 degrees Celsius. Activity reduced after 30 min at 80 degrees Celsius).

• ·Literature 1

• • Title

  • Purification and characterization of a novel protein from Peganum harmala seeds with antifungal, antiproliferation and anti-HIV-1 reverse transcriptase activities.

• • Pubmed ID

  • PubMed ID is not available

• • Reference

  • Submitted (MAR-2012) to UniProtKB

• • Author

  • Ma XJ, Liu DL, Tang HS, Wang Y, Sun SR.