General Information
-
DRAMP ID
- DRAMP00803
-
Peptide Name
- Palicourein (Cyclotides; Plants)
-
Source
- Palicourea condensata (Cappel)
-
Family
- Belongs to the cyclotide family
-
Gene
- Not found
-
Sequence
- GDPTFCGETCRVIPVCTYSAALGCTCDDRSDGLCKRN
-
Sequence Length
- 37
-
UniProt Entry
- P84645
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antiviral
-
Target Organism
-
- [Ref.18008336]Virus:HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=100 nM).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
-
- [Ref.11430013] Cytotoxicity: human T-lymphoblastoid cell line (CEM-SS)(EC50=0.10 Μm, IC50=1.5 μM) .
- [Ref.18008336]CEM-SS cells:IC50=1500 nM.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Cyclization (N termini to C termini)
-
C-terminal Modification
- Cyclization (N termini to C termini)
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys1 and Cys19; Cys5 and Cys21; Cys11 and Cys28;hydrogen bonds between the oxygen atoms of the Glu3 carboxyl group and the backbone amides of residues Thr12, Thr13, and Ser14, and the side chain of Ser14.
-
Stereochemistry
- L
-
Structure
- Combine helix and strand structure
-
Structure Description
- Palicourein has an atypical size and composition within one of the surface-exposed loops.
-
Helical Wheel Diagram
-
PDB ID
- 1R1F resolved by NMR.
- 1R1F-> 
-
Predicted Structure
- There is no predicted structure for DRAMP00803.
Physicochemical Information
-
Formula
- C159H256N48O56S6
Absent Amino Acids
- HMQW
Common Amino Acids
- C
Mass
- 3928.43
PI
- 4.78
Basic Residues
- 4
Acidic Residues
- 5
Hydrophobic Residues
- 8
Net Charge
- -1
-
Boman Index
- -74.98
Hydrophobicity
- -0.189
Aliphatic Index
- 52.7
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1865
Absorbance 280nm
- 51.81
Polar Residues
- 18
DRAMP00803
Comments Information
Function
- Probably participates in a plant defense mechanism. Inhibits the cytopathic effects of the human immunodeficiency virus.
In vitro activity
- Palicourein inhibits the cytopathic effects of HIV-1RF infection of CEM-SS cells with an EC50 value of 0.1 µM and an IC50 value of 1.5 µM.
PTM
- This is a cyclic peptide which may contain three disulfide bonds 6-24; 10-26; 16-34.
Literature Information
- ·Literature 1
-
Title
- A novel anti-HIV macrocyclic peptide from Palicourea condensata.
-
Pubmed ID
- 11430013
-
Reference
- J Nat Prod. 2001 Feb;64(2):249-250.
-
Author
- Bokesch HR, Pannell LK, Cochran PK, Sowder RC 2nd, McKee TC, Boyd MR.
- ·Literature 2
-
Title
- Solution structure of the cyclotide palicourein: implications for the development of a pharmaceutical framework.
-
Pubmed ID
- 14725768
-
Reference
- Structure. 2004 Jan;12(1):85-94.
-
Author
- Barry DG, Daly NL, Bokesch HR, Gustafson KR, Craik DJ.
- ·Literature 3
-
Title
- Cyclotides as natural anti-HIV agents.
-
Pubmed ID
- 18008336
-
Reference
- Biopolymers. 2008;90(1):51-60.
-
Author
- Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.