General Information
-
DRAMP ID
- DRAMP01352
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Peptide Name
- Tigerinin-3 (Frogs, amphibians, animals)
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Source
- Rana tigerina (Indian bull frog)
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Family
- Not found
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Gene
- Not found
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Sequence
- RVCYAIPLPICY
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Sequence Length
- 12
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UniProt Entry
- P82653
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- Gram-positive bacteria: Bacillus subtilis (MIC=30 µg/ml), Staphylococcus aureus ATCC 8530 (MIC=30 µg/ml), Micrococcus luteus MT 166 (MIC=30 µg/ml);
- Gram-negative bacteria: Escherichia coli (MIC=40 µg/ml), Pseudomonas putida NCIM 2102 (MIC=40 µg/ml).
- Fungi: Saccharomyces cerevisiae ATCC 8530 (MIC=80 µg/ml).
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Hemolytic Activity
-
- [Ref.11031261] It exhibits no hemolytic activity up to a concentration of 200 μg/ml against rat erythrocytes.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Cell membrane
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bond between Cys3 and Cys11.
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Stereochemistry
- L
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP01352.
Physicochemical Information
-
Formula
- C66H103N15O15S2
Absent Amino Acids
- DEFGHKMNQSTW
Common Amino Acids
- CIPY
Mass
- 1410.76
PI
- 8.05
Basic Residues
- 1
Acidic Residues
- 0
Hydrophobic Residues
- 5
Net Charge
- +1
-
Boman Index
- 7.97
Hydrophobicity
- 1.125
Aliphatic Index
- 130
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 3105
Absorbance 280nm
- 282.27
Polar Residues
- 4
DRAMP01352
Comments Information
Function
- Has antibacterial against Gram-positive and Gram-negative bacteria. Also has antifungal activity against C. albicans.
Literature Information
- ·Literature 1
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Title
- Tigerinins: novel antimicrobial peptides from the Indian frog Rana tigerina.
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Pubmed ID
- 11031261
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Reference
- J Biol Chem. 2001 Jan 26;276(4):2701-2707.
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Author
- Sai KP, Jagannadham MV, Vairamani M, Raju NP, Devi AS, Nagaraj R, Sitaram N.