General Information

    • DRAMP ID

    • DRAMP01371

    • Peptide Name

    • Odorranain-NR (Frogs, amphibians, animals)

    • Source

    • Odorrana grahami (Yunnanfu frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLLSGILGAGKHIVCGLTGCAKA

    • Sequence Length

    • 23

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.18282640]Gram-positive bacterium: Staphylococcus aureus ATCC 2592 (MIC=9.375 µg/ml);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC>100 µg/ml), Bacillus dysenteriae (MIC=37.5 µg/ml);
      • Yeast: Candida albicans ATCC 2002 (MIC=18.75 µg/ml).

    • Hemolytic Activity

      • [Ref:18282640] It has little hemolytic activity at 100 μg/ml against rabbit red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys15 and Cys20.

    • Stereochemistry

    • L

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • Odorranain-NR has a large proportion of beta-sheet. In Tris HCl + SDS, odorranain-NR has a major conformation of beta-sheet (79.2%) and a minor conformation of beta-turn (1.3%). In Tris HCl, the beta-sheet has been decreased to 57.1% and the beta-turn has been increased to 10.5%. The beta-sheet has been decreased to 39.8% in water.

    • Helical Wheel Diagram

    • DRAMP01371 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01371.

Physicochemical Information

    • Formula

    • C93H163N27O26S2

    • Absent Amino Acids

    • DEFMNPQRWY

    • Common Amino Acids

    • G

    • Mass

    • 2139.6

    • PI

    • 8.9

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +3

    • Boman Index

    • 25.46

    • Hydrophobicity

    • 1.039

    • Aliphatic Index

    • 127.39

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.68

    • Polar Residues

    • 10

DRAMP01371

DRAMP01371 chydropathy plot

Comments Information

    • Function

    • Odorranain-NR exhibits antimicrobial activity against the tested strains except E. coli ATCC25922. Odorranain-NR has little hemolytic activity on red blood cells even with peptide concentration up to 100 mg/ml.

    • PTM

    • Odorranain-NR has an intramolecular disulfide-bridged hexapeptide segment, which is a new type of disulfide-bridge motif in amphibian antimicrobial peptides.

Literature Information

  • ·Literature 1

    • Title

    • A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami.

    • Reference

    • Peptides. 2008 Apr;29(4):529-535.

    • Author

    • Che Q et al Lai R.