• • DRAMP ID

  • DRAMP02251

• • Peptide Name

  • Ranatuerin-2CSa (Frogs, amphibians, animals)

• • Source

  • Rana cascadae (Cascades frog)

• • Family

  • Belongs to the frog skin active peptide family (Brevinin subfamily)

• • Gene

  • Not found

• • Sequence

  • GILSSFKGVAKGVAKDLAGKLLETLKCKITGC

• • Sequence Length

  • 32

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.17451843]Gram-negative bacterium:Escherichia coli ATCC 25726 (MIC=5 µM);
    • Gram-positive bacterium: Staphylococcus aureus ATCC 25923 (MIC=10 µM)

• • Hemolytic Activity

  • • [Ref.17451843] LD50=150 μM against human erythrocytes

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Cyclization (Cys27 and Cys32)

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bond between Cys27 and Cys32.

• • Stereochemistry

  • L

• • Structure

  • Alpha helix (4 helices; 25 residues)

• • Structure Description

  • In a 2,2,2-trifluoroethanol (TFE-d(3))-H(2)O solvent mixture, the structure is characterised by a full length helix-turn-helix conformation between residues I(2)-L(21), L(22)-L(25) and K(26)-T(30), respectively.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 2K10 resolved by NMR.

• Predicted Structure

• There is no predicted structure for DRAMP02251.

DRAMP02251

• • Function

  • Has antibacterial activiy against Gram-negative and Gram-positive bacteria. Also has Hemolytic activity against human erythrocytes.

• ·Literature 1

• • Title

  • Peptide defenses of the Cascades frog Rana cascadae: implications for the evolutionary history of frogs of the Amerana species group.

• • Pubmed ID

  • 17451843

• • Reference

  • Peptides. 2007 Jun;28(6):1268-1274.

• • Author

  • Conlon JM, Al-Dhaheri A, Al-Mutawa E, Al-Kharrge R, Ahmed E, Kolodziejek J, Nowotny N, Nielsen PF, Davidson C.

• ·Literature 2

• • Title

  • Conformational analysis of the broad-spectrum antibacterial peptide, ranatuerin-2CSa: identification of a full length helix-turn-helix motif.

• • Pubmed ID

  • 18387372

• • Reference

  • Biochim Biophys Acta. 2008 Jun;1784(6):924-929.

• • Author

  • Subasinghage AP, Conlon JM, Hewage CM.