General Information

    • DRAMP ID

    • DRAMP02251

    • Peptide Name

    • Ranatuerin-2CSa (Frogs, amphibians, animals)

    • Source

    • Rana cascadae (Cascades frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GILSSFKGVAKGVAKDLAGKLLETLKCKITGC

    • Sequence Length

    • 32

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.17451843]Gram-negative bacterium:Escherichia coli ATCC 25726 (MIC=5 µM);
      • Gram-positive bacterium: Staphylococcus aureus ATCC 25923 (MIC=10 µM)

    • Hemolytic Activity

      • [Ref.17451843] LD50=150 μM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys27 and Cys32)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys27 and Cys32.

    • Stereochemistry

    • L

    • Structure

    • Alpha helix (4 helices; 25 residues)

    • Structure Description

    • In a 2,2,2-trifluoroethanol (TFE-d(3))-H(2)O solvent mixture, the structure is characterised by a full length helix-turn-helix conformation between residues I(2)-L(21), L(22)-L(25) and K(26)-T(30), respectively.

    • Helical Wheel Diagram

    • DRAMP02251 helical wheel diagram

    • PDB ID

    • 2K10 resolved by NMR.
  • 2K10-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C145H254N38O41S2

    • Absent Amino Acids

    • HMNPQRWY

    • Common Amino Acids

    • K

    • Mass

    • 3249.96

    • PI

    • 9.51

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • -2.58

    • Hydrophobicity

    • 0.444

    • Aliphatic Index

    • 112.81

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.03

    • Polar Residues

    • 11

DRAMP02251

DRAMP02251 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activiy against Gram-negative and Gram-positive bacteria. Also has Hemolytic activity against human erythrocytes.

Literature Information

  • ·Literature 1

    • Title

    • Peptide defenses of the Cascades frog Rana cascadae: implications for the evolutionary history of frogs of the Amerana species group.

    • Reference

    • Peptides. 2007 Jun;28(6):1268-1274.

    • Author

    • Conlon JM, Al-Dhaheri A, Al-Mutawa E, Al-Kharrge R, Ahmed E, Kolodziejek J, Nowotny N, Nielsen PF, Davidson C.
  • ·Literature 2

    • Title

    • Conformational analysis of the broad-spectrum antibacterial peptide, ranatuerin-2CSa: identification of a full length helix-turn-helix motif.

    • Reference

    • Biochim Biophys Acta. 2008 Jun;1784(6):924-929.

    • Author

    • Subasinghage AP, Conlon JM, Hewage CM.