General Information

    • DRAMP ID

    • DRAMP02257

    • Peptide Name

    • Ranatuerin-2P (Ranatuerin 2P; Frogs, amphibians, animals)

    • Source

    • Rana pipiens (Northern leopard frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • LMDTVKNVAKNLAGHMLDKLKCKITGC

    • Sequence Length

    • 27

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal,Antiviral

    • Target Organism

      • [Ref.10651828] Gram-positive bacteria: Staphylococcus aureus (MIC=50 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=13 µM);
      • Yeast: Candida albicans (MIC=67 µM).
      • [Ref.11601906]Virus:
      • Frog Virus 3: 90% inhibition at 500 µM;
      • Channel Catfish Virus: 99% inhibition at 50 µM.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization of a C-terminal Cys residue (forming a disulfide bond)

    • Nonterminal Modifications and Unusual Amino Acids

    • There is a disulfide bond between Cys22 and Cys27.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02257 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02257.

Physicochemical Information

    • Formula

    • C126H222N36O36S4

    • Absent Amino Acids

    • EFPQRSWY

    • Common Amino Acids

    • K

    • Mass

    • 2945.61

    • PI

    • 9.24

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +4

    • Boman Index

    • -22.83

    • Hydrophobicity

    • 0.059

    • Aliphatic Index

    • 101.11

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.81

    • Polar Residues

    • 8

DRAMP02257

DRAMP02257 chydropathy plot

Comments Information

    • Function

    • Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi.

Literature Information

  • ·Literature 1

    • Title

    • Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin.

    • Reference

    • Virology. 2001 Sep 30;288(2):351-7.

    • Author

    • Chinchar VG, Wang J, Murti G, Carey C, Rollins-Smith L.
  • ·Literature 2

    • Title

    • Peptides with antimicrobial activity from four different families isolated from the skins of the North American frogs Rana luteiventris, Rana berlandieri and Rana pipiens.

    • Reference

    • Eur J Biochem. 2000 Feb;267(3):894-900.

    • Author

    • Goraya J, Wang Y, Li Z, O'Flaherty M, Knoop FC, Platz JE, Conlon JM.