• DRAMP ID

    • DRAMP02295
    • Peptide Name

    • Gaegurin-5 (Gaegurin 5; GGN5; Brevinin-1EMa; Frogs, amphibians, animals)
    • Source

    • Glandirana rugosa (Japanese wrinkled frog) (Rana rugosa)
    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)
    • Gene

    • GGN5
    • Sequence

    • FLGALFKVASKVLPSVFCAITKKC
    • Sequence Length

    • 24
    • Protein Existence

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiprotozoal
    • Target Organism

      • [Ref.7999137]Gram-positive bacteria: Micrococcus luteus (MIC=2.5 µg/ml), Staphylococcus epidermidis (MIC=10 µg/ml), Bacillus subtilis (MIC=10 µg/ml);
      • Gram-negative bacteria: Klebsiella pneumoniae (MIC=50 µg/ml), Shigella dysentariae (MIC=50 µg/ml), Pseudomonas putida (MIC=50 µg/ml), Pseudomonas aeruginosa (MIC=100 µg/ml), Eshchericia coli (MIC=50 µg/ml);
      • Fungi: Saccharomyces cerevisiae (MIC=50 µg/ml), Candida albicans (MIC=50 µg/ml), Salmonella typhimurium (MIC=200 µg/ml), Proteus mirabilis (MIC>200 µg/ml).
    • Hemolytic Activity

      • [Ref.7999137] It has 0.19% hemolytic activity at 0.1 μg/ml, 0.31% hemolytic activity at 1 μg/ml, 0.78% hemolytic activity at 10 μg/ml, 1.01% hemolytic activity at 100 μg/ml against human red blood cells
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)
    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP02295 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP02295.
    • Formula

    • C122H200N28O28S2
    • Absent Amino Acids

    • DEHMNQRWY
    • Common Amino Acids

    • K
    • Mass

    • 2571.22
    • PI

    • 9.7
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 13
    • Net Charge

    • +4
    • Boman Index

    • 18.1
    • Hydrophobicity

    • 1.142
    • Aliphatic Index

    • 113.75
    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 125
    • Absorbance 280nm

    • 5.43
    • Polar Residues

    • 6

DRAMP02295

DRAMP02295 chydropathy plot
    • Function

    • Has low hemolytic activity. Has a broad spectrum of activity against both Gram-positive and Gram-negative bacteria, fungi and protozoa. PTM
  • ·Literature 1
    • Title

    • Antimicrobial peptides from the skin of a Korean frog, Rana rugosa.
    • Reference

    • Biochem Biophys Res Commun. 1994 Nov 30;205(1):948-954.
    • Author

    • Park JM, Jung JE, Lee BJ.