General Information

    • DRAMP ID

    • DRAMP03823

    • Peptide Name

    • Dermaseptin derivative K4-S4-(1-13)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ALWKTLLKKVLKA

    • Sequence Length

    • 13

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.15917541]Gram-negative bacterium: Escherichia coli U16318 (MIC=9-3 µM);
      • Gram-positive bacterium: Staphylococcus aureus B38302 (MIC=4.5-1.5 µM).

    • Hemolytic Activity

      • [Ref.15917541]LC50=50 μM against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix (1 helices; 5 residues)

    • Structure Description

    • The Ramachandran plot of P showes that residues 2 and 4-13 are all in α-helix regions of the plot; the residues in all of the structures are in the core α-helix region for residues 2, 3, and 6-11, and both residue 5 and 12 had over 60% in the core and the rest in allowed α-helix regions.

    • Helical Wheel Diagram

    • DRAMP03823 helical wheel diagram

    • PDB ID

    • 2DD6 resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP03823.

Physicochemical Information

    • Formula

    • C74H130N18O15

    • Absent Amino Acids

    • CDEFGHIMNPQRSY

    • Common Amino Acids

    • KL

    • Mass

    • 1511.96

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +4

    • Boman Index

    • 4.9

    • Hydrophobicity

    • 0.446

    • Aliphatic Index

    • 157.69

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 1

DRAMP03823

Comments Information

    • Function

    • Has antibacterial activity. Also has hemolytic activity against red blood cells (RBCs).

Literature Information

  • ·Literature 1

    • Title

    • Consequences of N-acylation on structure and membrane binding properties of dermaseptin derivative K4-S4-(1-13).

    • Reference

    • J Biol Chem. 2006 Apr 7;281(14):9432-9438.

    • Author

    • Shalev DE, Rotem S, Fish A, Mor A.
  • ·Literature 2

    • Title

    • Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides.

    • Reference

    • Antimicrob Agents Chemother. 2005 Jun;49(6):2412-2420.

    • Author

    • Radzishevsky IS, Rotem S, Zaknoon F, Gaidukov L, Dagan A, Mor A.