• DRAMP ID

    • DRAMP03827
    • Peptide Name

    • Novispirin G-10 (mutation of Ovispirin-1)
    • Source

    • Synthetic construct
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • KNLRRIIRKGIHIIKKYG
    • Sequence Length

    • 18
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic
    • Biological Activity

    • Antibacterial, Cytotoxic, Anti-Gram+, Anti-Gram-, Antimicrobial
    • Target Organism

      • [Ref.11932493]Gram-negative bacteria: Pseudomonas aeruginosa 9 strains (MIC=0.11-12.7 µg/ml), Stenotrophomonas maltophilia 5 strains (MIC=0.29-3.6 µg/ml);
      • Gram-positive bacteria: Listeria monocytogenes EGD [n=3] (MIC=1.4±0.4 µg/ml), Staphylococcus aureus 930918 [n=3] (MIC=4.6±1.7 µg/ml).
    • Hemolytic Activity

      • [Ref.11932493]1.8% hemolytic at 40 µg/mL, 2.5% hemolytic activity at 80 µg/mL against human red blood cells
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Free
    • Nonterminal Modifications and Unusual Amino Acids

    • Free
    • Stereochemistry

    • L
    • Structure

    • Alpha helix (2 helices; 11 residues)
    • Structure Description

    • Novispirin G-10 shows an α-helix-hinge-310 helix structural motif with residues 4-11 forming the α-helix, residues 12 and 13 forming the hinge and residues 14-16 forming the 310 helix.
    • Helical Wheel Diagram

    • DRAMP03827 helical wheel diagram
    • PDB ID

    • 1HU6 resolved by NMR.
    • Predicted Structure

    • There is no predicted structure for DRAMP03827.
    • Formula

    • C101H180N34O21
    • Absent Amino Acids

    • ACDEFMPQSTVW
    • Common Amino Acids

    • I
    • Mass

    • 2206.76
    • PI

    • 11.75
    • Basic Residues

    • 8
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 6
    • Net Charge

    • +8
    • Boman Index

    • -47
    • Hydrophobicity

    • -0.644
    • Aliphatic Index

    • 130
    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 1490
    • Absorbance 280nm

    • 87.65
    • Polar Residues

    • 4

DRAMP03827

DRAMP03827 chydropathy plot
    • Function

    • Possess potently antimicrobial activity. Also has hemolytic activity against Human red blood cells, and has cytotoxicity activity against ME-180 cervical cells (33.2% at 200 µg/ml, 5.0% at 50 µg/ml, 7.1% at 25 µg/ml) and A-549 pulmonary cells (33.2% at 200 µg/ml, 14.6% at 50 µg/ml, 10.0% at 25 µg/ml).
  • ·Literature 1
    • Title

    • Impact of single-residue mutations on the structure and Function: of ovispirin/novispirin antimicrobial peptides.
    • Reference

    • Protein Eng. 2002 Mar;15(3):225-232.
    • Author

    • Sawai MV, Waring AJ, Kearney WR, McCray PB Jr, Forsyth WR, Lehrer RI, Tack BF.