General Information

    • DRAMP ID

    • DRAMP03976

    • Peptide Name

    • N-4L (analog of P18)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • WFKKIPKFLHLLKKF

    • Sequence Length

    • 15

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antitumor

    • Target Organism

      • Gram-negative bacteria: Escherichia coli KCTC 1682 (MIC=3.125 µM), Pseudomonas aeruginosa KCTC 1637 (MIC=1.56 µM), Proteus vulgaris (MIC=12.5 µM).
      • Gram-positive bacteria: Bacillus subtilis KCTC 1918 (MIC=0.78 µM), Staphylococcus aureus KCTC 1621 (MIC=3.125 µM), Bacillus megaterium KCTC 1096 (MIC=0.78 µM).
      • Tumor cells: MDA-MB-361 (IC50=5.0 µM), Jurkat (IC50=6.0 µM), K-562 (IC50=3.0 µM), Normal cells NIH 3T3 (IC50=50.0 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP03976 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP03976.

Physicochemical Information

    • Formula

    • C103H157N23O16

    • Absent Amino Acids

    • ACDEGMNQRSTVY

    • Common Amino Acids

    • K

    • Mass

    • 1973.52

    • PI

    • 10.6

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +6

    • Boman Index

    • -1.46

    • Hydrophobicity

    • -0.06

    • Aliphatic Index

    • 104

    • Half Life

      • Mammalian:2.8 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 392.86

    • Polar Residues

    • 0

DRAMP03976

Comments Information

    • Function

    • Has antibacterial activiy against Gram-negative and Gram-positive bacteria. Also has antitumor activity.

Literature Information

  • ·Literature 1

    • Title

    • Antibacterial, antitumor and hemolytic activities of alpha-helical antibiotic peptide, P18 and its analogs.

    • Reference

    • J Pept Res. 2001 Dec;58(6):504-514.

    • Author

    • Shin SY, Lee SH, Yang ST, Park EJ, Lee DG, Lee MK, Eom SH, Song WK, Kim Y, Hahm KS, Kim JI.