General Information

    • DRAMP ID

    • DRAMP20778

    • Peptide Name

    • Temporin-SHf (frogs,amphibians,animals)

    • Source

    • Pelophylax saharica(Sahara frog)

    • Family

    • Belongs to the Temporin family

    • Gene

    • Not found

    • Sequence

    • FFFLSRIF

    • Sequence Length

    • 8

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.20308076] Gram-negative bacteria: Escherichia coli ATCC 25922(MIC=25 μM); Escherichia coli ATCC 35218(MIC>200 μM); Escherichia coli D21(MIC=100 μM); Escherichia coli ML-35p(MIC=30 μM); Pseudomonas aeruginosa ATCC 27853(MIC>200 μM);
      • Gram-positive bacteria: Staphylococcus aureus ATCC 25923(MIC=12.5 μM); E. faecalis ATCC 29212(MIC=50 μM); Bacillus megaterium(MIC=3 μM);
      • Yeasts: Candida albicans ATCC 90028(MIC=50 μM); C. parapsilosis ATCC 22019(MIC=50 μM); S. cerevisiae(MIC=12.5 μM);
      • Fungus(MIC>200 μM)

    • Hemolytic Activity

      • [Ref.20308076] Temporin-SHf is not cytotoxic against human erythrocytes (LC50 = 200 μm), with a hemolytic activity detected only at peptide concentration far above the MIC values determined for the sensitive strains (3–50 μm)

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Once bound parallel to the microbial membrane surface, it folds into a non-amphipathic hydrophobic

    • Helical Wheel Diagram

    • DRAMP20778 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20778.

Physicochemical Information

    • Formula

    • C57H77N11O10

    • Absent Amino Acids

    • ACDEGHKMNPQTVWY

    • Common Amino Acids

    • F

    • Mass

    • 1076.31

    • PI

    • 9.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +1

    • Boman Index

    • 344

    • Hydrophobicity

    • 1.775

    • Aliphatic Index

    • 97.5

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP20778

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative bacteria (Escherichia coli ATCC 25922, Escherichia coli ATCC 35218, Escherichia coli D21, Escherichia coli ML-35p, Pseudomonas aeruginosa ATCC 27853) and Gram-positive bacteria (Staphylococcus aureus ATCC 25923, E. faecalis ATCC 29212, Bacillus megaterium. Antifungal activity)

Literature Information

  • ·Literature 1

    • Title

    • Temporin-SHf, a new type of phe-rich and hydrophobic ultrashort antimicrobial peptide.

    • Reference

    • J Biol Chem. 2010 May 28;285(22):16880-92.

    • Author

    • Abbassi F, Lequin O, Piesse C, Goasdou