General Information
-
DRAMP ID
- DRAMP20784
-
Peptide Name
- Megin 1
-
Source
- Amphibian skin
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- FLKGCWTKWYSLKPKCPF
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
-
Protein Existence
- Transcript level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
-
Target Organism
-
- [Ref.27114317] Gram-negative bacteria: Escherichia coli (ATCC 25922)(MIC=25μg/mL);B. dysenteriae(MIC=3μg/mL);
- Gram-positive bacteria:Staphylococcus aureus (ATCC 2592)(MIC=6.25μg/mL);Bacillus subtilis (ATCC 6633)(MIC=3μg/mL) ;
- Fungi:C.albicans (ATCC 2002)(MIC=50μg/mL)
-
Hemolytic Activity
-
- [Ref.27114317] Human red blood cells: 2.3% hemolysis at 25μg/ml; 5.6% hemolysis at 50μg/ml; 18.7% hemolysis at 100μg/ml; 29.5% hemolysis at 200μg/ml. Rabbit red blood cells :3.1% hemolysis at 25μg/ml; 7.3% hemolysis at 50μg/ml; 20.5% hemolysis at 100μg/ml; 32.9% hemolysis at 200μg/ml
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bond between Cys5 and Cys16
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- The peptide contains an intra-molecular di-sulfide bridge
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20784.
Physicochemical Information
-
Formula
- C110H158N24O22S2
Absent Amino Acids
- ADEHIMNQRV
Common Amino Acids
- K
Mass
- 2232.73
PI
- 9.6
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 6
Net Charge
- +4
-
Boman Index
- -435
Hydrophobicity
- -0.311
Aliphatic Index
- 43.33
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 12615
Absorbance 280nm
- 742.06
Polar Residues
- 6
DRAMP20784
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Two novel antimicrobial peptides from skin venoms of spadefoot toad Megophrys minor.
-
Pubmed ID
- 27114317
-
Reference
- Chin J Nat Med. 2016 Apr;14(4):294-298.
-
Author
- Yang HL, Shen ZQ, Liu X, Kong Y.