• • DRAMP ID

  • DRAMP20785

• • Peptide Name

  • Megin 2

• • Source

  • Amphibian skin

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • FFVLKFLLKWAGKVGLEHLACKFKNWC

• • Sequence Length

  • 27

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Transcript level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.27114317] Gram-negative bacteria: Escherichia coli (ATCC 25922)(MIC=6.25μg/mL);B. dysenteriae(MIC=1.5μg/mL);
    • Gram-positive bacteria:Staphylococcus aureus (ATCC 2592)(MIC=12.5μg/mL);Bacillus subtilis (ATCC 6633)(MIC=1.5μg/mL) ;
    • Fungi:C.albicans (ATCC 2002)(MIC=12.5μg/mL)

• • Hemolytic Activity

  • • [Ref.27114317] Human red blood cells: 3.9% hemolysis at 25μg/ml; 13.7% hemolysis at 50μg/ml; 27.7% hemolysis at 100μg/ml; 45.3% hemolysis at 200μg/ml;Rabbit red blood cells: 5.6% hemolysis at 25μg/ml; 15.2% hemolysis at 50μg/ml; 36.2% hemolysis at 100μg/ml; 50.3% hemolysis at 200 μg/ml

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bond between Cys21 and Cys27.

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • The peptide contains an intra-molecular di-sulfide bridge

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP20785.

DRAMP20785

• • Function

  • Antibacterial activity against the Gram-positive and Gram-negative bacteria.

• ·Literature 1

• • Title

  • Two novel antimicrobial peptides from skin venoms of spadefoot toad Megophrys minor.

• • Pubmed ID

  • 27114317

• • Reference

  • Chin J Nat Med. 2016 Apr;14(4):294-298.

• • Author

  • Yang HL, Shen ZQ, Liu X, Kong Y.