General Information
-
DRAMP ID
- DRAMP20786
-
Peptide Name
- mini-ChBac7.5N alpha
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Source
- Capra hircus
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- RRLRPRRPRLPRPRPRPRPRPR
-
Sequence Length
- 22
-
UniProt Entry
- No entry found
-
Protein Existence
- Transcript level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.27795854] Gram-negative bacteria: E.coli (ML35p)(MIC=0.3 ± 0.1μM without NaCl;MIC=1.5 ± 0.2μM 100 mM NaCl;MIC=1μM Broth microdilution assay);E.coli (ATCC 25922)(MIC=0.6 ± 0.1μM without NaCl;MIC=0.9 ± 0.2μM 100 mM NaCl;MIC=2μM Broth microdilution assay);E.coli (M17)(MIC=0.5 ± 0.1μM without NaCl;MIC=0.8 ± 0.1μM 100 mM NaCl;MIC=2μM Broth microdilution assay);Pseudomonas aeruginosa (ATCC 27853)(MIC=1.1 ± 0.4μM without NaCl;MIC=3.7 ± 1.2μM 100 mM NaCl;MIC=2μM Broth microdilution assay);Pseudomonas aeruginosa clinical isolate(MIC=2μM Broth microdilution assay);Klebsiella spp. clinical isolate(MIC=4μM Broth microdilution assay);Acinetobacter baumannii clinical isolate(MIC=2μM Broth microdilution assay);
- Gram-positive bacteria:Listeria monocytogenes (EGD)(MIC=0.2 ± 0.1μM without NaCl;MIC=1.0 ± 0.2μM 100 mM NaCl;MIC=2μM Broth microdilution assay);Micrococcus luteus CIP A270(MIC=1μM Broth microdilution assay);Staphylococcus aureus (710A)(MIC=0.7 ± 0.2μM without NaCl;MIC> 50μM 100 mM NaCl;MIC>64μM Broth microdilution assay);Staphylococcus aureus (ATCC 25923)(MIC>64μM Broth microdilution assay);MRSA (ATCC 33591)(MIC=0.7 ± 0.2μM without NaCl;MIC> 50μM 100 mM NaCl;MIC>64μM Broth microdilution assay);Staphylococcus intermedius clin. isolate(MIC>64μM Broth microdilution assay);
- Fungi:Candida albicans (820)(MIC=0.3 ± 0.1μM without NaCl;MIC> 50μM 100 mM NaCl;MIC=64μM Broth microdilution assay);Candida parapsilosis clinical isolate(MIC>64μM Broth microdilution assay)
-
Hemolytic Activity
-
- [Ref.27818338] No hemolytic activity
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Cytotoxicity
-
- [Ref.27795854] The cytotoxicity values of the experimental samples are not significantly different from the values of the control samples over the entire range of concentrations:1-30 μM against various types of cultured human cells: namly, reythroleukemi
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20786.
Physicochemical Information
-
Formula
- C124H224N58O23
Absent Amino Acids
- ACDEFGHIKMNQSTVWY
Common Amino Acids
- R
Mass
- 2895.52
PI
- 13.04
Basic Residues
- 12
Acidic Residues
- 0
Hydrophobic Residues
- 2
Net Charge
- +12
-
Boman Index
- -16920
Hydrophobicity
- -2.691
Aliphatic Index
- 35.45
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 0
DRAMP20786
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria.
Literature Information
- ·Literature 1
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Title
- Minibactenecins ChBac7.N
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Pubmed ID
- 27795854
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Reference
- Acta Naturae. 2016 Jul-Sep;8(3):136-146.
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Author
- Shamova OV, Orlov DS, Zharkova MS, Balandin SV, Yamschikova EV, Knappe D, Hoffmann R, Kokryakov VN, Ovchinnikova TV.