General Information
-
DRAMP ID
- DRAMP20801
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Peptide Name
- Mussel Defensin MGD-1 (Mediterranean mussel defensin 1; mollusca/molluscs/mollusks, invertebrates, animals)
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Source
- Mytilus galloprovincialis (Mediterranean mussel)
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Family
- Belongs to the invertebrate defensin family. Type 2 subfamily.
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Gene
- FH3
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Sequence
- GFGCPNNYQCHRHCKSIPGRCGGYCGGWHRLPCTCYRC
-
Sequence Length
- 38
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UniProt Entry
- P80571
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.8925841] Gram-positive bacteria: Micrococcus luteus (MIC=0.08 μg/ml);
- Gram-negative bacteria: Escherichia coli (MIC=6.4 μg/ml), Vibrio alginolyticus (MIC=6.4 μg/ml), Vibrio splendidus (MIC=1.6 μg/ml), Vibrio P1 (MIC=6.4 μg/ml).
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Hemolytic Activity
-
- [Ref.8925841] The plasma is capable of causing 20% of the total hemolysis.
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Cytotoxicity
-
- [Ref.8925841] The protozoan P. marinus is not killed by pure peptide, even at a concentration of 16 μg/ml.
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Binding Target
- Cell membranes
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys4 and Cys25, Cys10 and Cys33, Cys14 and Cys35, Cys21 and Cys38. Hydroxylation of Trp28 (3hydroxytryptophan)
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Stereochemistry
- L
-
Structure
- Combine Helix and Beta structure
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Structure Description
- The signature of MGD-1 is that of the arthropod defensins which range in length from 34 to 51 amino acid residues with 6 conserved cysteines engaged in three intramolecular disulfide bridges. They are characterized by the presence of a flexible amino-terminal loop, a central amphipathic
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Helical Wheel Diagram
-
PDB ID
- 1FJN resolved by NMR
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Predicted Structure
- There is no predicted structure for DRAMP20801.
Physicochemical Information
-
Formula
Absent Amino Acids
Common Amino Acids
Mass
- 4291.94
PI
- 8.94
Basic Residues
Acidic Residues
Hydrophobic Residues
Net Charge
-
Boman Index
- 0
Hydrophobicity
- 0
Aliphatic Index
- 0
Half Life
-
- Mammalian:
- Yeast:
- E.coli:
Extinction Coefficient Cystines
Absorbance 280nm
- 0
Polar Residues
DRAMP20801
Comments Information
Function
- Active against both Gram-positive and Gram-negative bacteria but is not cytotoxic towards human erythrocytes or protozoa.
Literature Information
- ·Literature 1
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Title
- A member of the arthropod defensin family from edible Mediterranean mussels (Mytilus galloprovincialis)
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Pubmed ID
- 8925841
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Reference
- Eur J Biochem. 1996 Aug 15;240(1):302-6.
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Author
- Hubert F, Noel T, Roch P.