General Information

    • DRAMP ID

    • DRAMP20808

    • Peptide Name

    • RI21 (PMAP-36 peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from PMAP-36

    • Gene

    • Not found

    • Sequence

    • RRLRKKTRKRLKKIGKVLKWI

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.27251456] Gram-positive bacteria: Staphylococcus aureus 29213 (MIC=4 μM, MBC=4 μM), Staphylococcus aureus 25923 (MIC=4 μM, MBC=4 μM), Staphylococcus epidermidis 12228 (MIC=4 μM, MBC=8 μM), Bacillus subtilis 63501 (MIC=1 μM, MBC=4 μM);
      • Gram-negative bacteria: Escherichia coli 25922 (MIC=2 μM, MBC=8 μM), Escherichia coli EC183 (MIC=1 μM, MBC=2 μM), Escherichia coli 1005 (MIC=2 μM, MBC=2 μM), Salmonella typhimurium C77-31 (MIC=1 μM, MBC=2 μM);
      • Fungi: Candida albicans 2.2086 (MIC=8 μM, MFC=16 μM), Candida albicans CA276 (MIC=8 μM, MFC=32 μM), Candida tropicalis 2.1975 (MIC=1 μM, MFC=2 μM), Candida krusei 2.1857 (MIC=2 μM, MFC=4 μM).

    • Hemolytic Activity

      • [Ref.27251456] 5% hemolysis at 32 μM, MHC=32 μM against human red blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20808 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20808.

Physicochemical Information

    • Formula

    • C124H230N44O23

    • Absent Amino Acids

    • ACDEFHMNPQSY

    • Common Amino Acids

    • K

    • Mass

    • 2705.47

    • PI

    • 12.61

    • Basic Residues

    • 12

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +12

    • Boman Index

    • -8411

    • Hydrophobicity

    • -1.295

    • Aliphatic Index

    • 106.67

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 275

    • Polar Residues

    • 2

DRAMP20808

DRAMP20808 chydropathy plot

Comments Information

    • Function

    • Antimicrobial activity against Gram-negative bacteria, Gram-positive bacteria and Fungi..

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial activity, improved cell selectivity and mode of action of short PMAP-36-derived peptides against bacteria and Candida.

    • Reference

    • Sci Rep. 2016 Jun 2;6:27258.

    • Author

    • Lyu Y, Yang Y, Lyu X, Dong N, Shan A.