General Information

    • DRAMP ID

    • DRAMP20816

    • Peptide Name

    • K1K8

    • Source

    • Synthetic construct

    • Family

    • Belongs to the Hp1404 family

    • Gene

    • Not found

    • Sequence

    • KILGKLWKGVKSIF

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.26952110] Gram-positive bacteria:Staphylococcus aureus AB94004 (MIC=6.25μg/mL), Staphylococcus aureus ATCC6538 (MIC=12.5μg/mL), Staphylococcus aureus ATCC25923 (MIC=12.5μg/mL), Staphylococcus aureus MRSA P1381 (MIC=6.25μg/mL), Staphylococcus aureus MRSA P1374 (MIC=6.25μg/mL);
      • Gram-negative bacteria: Escherichia coli AB94012 (MIC=25μg/mL), Escherichia coli ATCC25922 (MIC=50μg/mL), Pseudomonas aeruginosa AB93066 (MIC=50μg/mL), Pseudomonas aeruginosa ATCC9027 (MIC=50μg/mL)

    • Hemolytic Activity

      • [Ref.26952110] 10% hemolysis at 400μg/mL against human red blood cells

    • Cytotoxicity

      • [Ref.26952110] The cell vaibility of HFF cell lines is 100%, 108%, 105% and 38% at peptide concentrations of 0 μg/ml, 50 μg/ml, 100 μg/ml and 200 μg/ml.
      • The cell viability of HEK293T cell lines is 105%, 125%, 101% and 21% at peptide concentrations of

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20816 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20816.

Physicochemical Information

    • Formula

    • C80H133N19O16

    • Absent Amino Acids

    • ACDEHMNPQRTY

    • Common Amino Acids

    • K

    • Mass

    • 1617.05

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • 531

    • Hydrophobicity

    • 0.393

    • Aliphatic Index

    • 132.14

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 423.08

    • Polar Residues

    • 3

DRAMP20816

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative and Gram-positive bacteria

Literature Information

  • ·Literature 1

    • Title

    • K1K8: an Hp1404-derived antibacterial peptide

    • Reference

    • Appl Microbiol Biotechnol. 2016 Jun;100(11):5069-77.

    • Author

    • Li Z, Liu G, Meng L, Yu W, Xu X, Li W, Wu Y, Cao Z.