General Information

    • DRAMP ID

    • DRAMP20822

    • Peptide Name

    • FV-MA (FV7 and MA(Magainin 2 (9-21)) hybrid peptide)

    • Source

    • Synthetic construct

    • Family

    • FV7 and MA(Magainin 2 (9-21)) hybrid peptide

    • Gene

    • Not found

    • Sequence

    • FRIRVRVAKKFGKAFVGEIM

    • Sequence Length

    • 20

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.28178190] Gram-positive bacteria: Staphylococcus aureus ATCC 29213 (MIC=8 μM), Streptococcus Faecalis ATCC 29212 (MIC=16 μM), Bacillus subtilis CMCC 63501 (MIC=4 μM), Staphylococcus epidermidis ATCC 12228 (MIC=8 μM);
      • Gram-negative bacteria:Escherichia coli ATCC 25922 (MIC=8 μM), Escherichia coli UB 1005 (MIC=8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=8 μM), Pseudomonas aeruginosa PAO1 (MIC=2 μM), Salmonella typhimurium ATCC 14028 (MIC=4 μM), Salmonella typhimurium ATCC 7731 (MIC=4 μM).

    • Hemolytic Activity

      • [Ref.28178190] 1% hemolysis at 2 μM, 8% hemolysis at 4 μM, 10% hemolysis at 8 μM, 15% hemolysis at 16 μM, 20% hemolysis at 32 μM, 37% hemolysis at 64 μM, 55% hemolysis at 128 μM against human red cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix, random coil

    • Structure Description

    • The hybrid peptide displayed an unordered conformation in phosphate buffer.

    • Helical Wheel Diagram

    • DRAMP20822 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20822.

Physicochemical Information

    • Formula

    • C110H182N32O23S

    • Absent Amino Acids

    • CDHLNPQSTWY

    • Common Amino Acids

    • FKRV

    • Mass

    • 2352.92

    • PI

    • 11.73

    • Basic Residues

    • 6

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +5

    • Boman Index

    • -2947

    • Hydrophobicity

    • 0.3

    • Aliphatic Index

    • 92.5

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP20822

DRAMP20822 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative and Gram-positive bacteria

Literature Information

  • ·Literature 1

    • Title

    • High Specific Selectivity and Membrane-Active Mechanism of Synthetic Cationic Hybrid Antimicrobial Peptides Based on the Peptide FV7

    • Reference

    • Int J Mol Sci. 2017 Feb 6;18(2).

    • Author

    • Tan T, Wu D, Li W, Zheng X, Li W, Shan A.