• • DRAMP ID

  • DRAMP20927

• • Peptide Name

  • IN2(designed based on indolicidin and ranalexin)

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • RRPWRWPWWPWRR

• • Sequence Length

  • 13

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.26046345] Gram-negative bacteria: Escherichia coli ATCC 25922(MIC=31.25μg/ml), Pseudomonas aeruginosa ATCC 15442(MIC=31.25μg/ml), Acinetobacter baumanii ATCC 15308(MIC>250μg/ml), citrobacter spp(MIC>250μg/ml), Klebsiella pneumoniae(MIC>250μg/ml) ;
    • Gram-positive bacteria: Streptococcus pneumoniae(30 clinical isolates)(MIC=31.25–62.5μg/ml), Staphylococcus aureus ATCC 25923(MIC=31.25μg/ml), Methicillin resistant Staphylococcus aureus (MRSA)(MIC=31.25μg/ml), Enterococcus cloacae(MIC>250μg/ml),

• • Hemolytic Activity

  • • [Ref.26046345] IN2 showed 15.39% hemolytic activity at concentration of 250μg/ml against human RBCs.

• • Cytotoxicity

  • • [Ref.26046345] The cell viability of NL-20 cell line is not affected (<10%) below 62.5 μg/ml. The cell viability of NL-20 is 40% and 19% at peptide concentrations of 125 and 250 μg/ml.
    • The cell viability of WRL-68 cell line is not affected (<10%) bel

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP20927.

DRAMP20927

• • Function

  • Exhibits antimicrobial activity against the Gram-positive and Gram-negative bacteria.

• ·Literature 1

• • Title

  • Antimicrobial Activity of Novel Synthetic Peptides Derived from Indolicidin and Ranalexin against Streptococcus pneumoniae.

• • Pubmed ID

  • 26046345

• • Reference

  • PLoS One. 2015 Jun 5;10(6):e0128532.

• • Author

  • Jindal HM, Le CF, Mohd Yusof MY, Velayuthan RD, Lee VS, Zain SM, Isa DM, Sekaran SD.