General Information
-
DRAMP ID
- DRAMP20928
-
Peptide Name
- IN3(designed based on indolicidin and ranalexin)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- RRPWRWPRWPWRR
-
Sequence Length
- 13
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.26046345] Gram-negative bacteria: Escherichia coli ATCC 25922(MIC=31.25μg/ml), Pseudomonas aeruginosa ATCC 15442(MIC=31.25μg/ml), Acinetobacter baumanii ATCC 15308(MIC>250μg/ml), citrobacter spp(MIC>250μg/ml), Klebsiella pneumoniae(MIC>250μg/ml) ;
- Gram-positive bacteria: Streptococcus pneumoniae(30 clinical isolates)(MIC=62.5μg/ml), Staphylococcus aureus ATCC 25923(MIC=31.25μg/ml), Methicillin resistant Staphylococcus aureus (MRSA)(MIC=62.5μg/ml), Enterococcus cloacae(MIC>250μg/ml),
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Hemolytic Activity
-
- [Ref.26046345] The hemolytic activities of IN3 is 2.39% at 250μg/ml against human RBCs.
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Cytotoxicity
-
- [Ref.26046345] The cell viability of NL-20 cell line is not affected (<10%) below 62.5 μg/ml. The cell viability of NL-20 is 93% and 26% at peptide concentrations of 125 and 250 μg/ml.
- The cell viability of WRL-68 cell line is not affected (<10%) bel
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20928.
Physicochemical Information
-
Formula
- C95H135N35O14
Absent Amino Acids
- ACDEFGHIKLMNQSTVY
Common Amino Acids
- R
Mass
- 1991.34
PI
- 12.7
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 4
Net Charge
- +6
-
Boman Index
- -8020
Hydrophobicity
- -2.723
Aliphatic Index
- 0
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 22000
Absorbance 280nm
- 1833.33
Polar Residues
- 0
DRAMP20928
Comments Information
Function
- Exhibits antimicrobial activity against the Gram-positive and Gram-negative bacteria.
Literature Information
- ·Literature 1
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Title
- Antimicrobial Activity of Novel Synthetic Peptides Derived from Indolicidin and Ranalexin against Streptococcus pneumoniae.
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Pubmed ID
- 26046345
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Reference
- PLoS One. 2015 Jun 5;10(6):e0128532.
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Author
- Jindal HM, Le CF, Mohd Yusof MY, Velayuthan RD, Lee VS, Zain SM, Isa DM, Sekaran SD.