• DRAMP ID

    • DRAMP20928
    • Peptide Name

    • IN3(designed based on indolicidin and ranalexin)
    • Source

    • Synthetic construct
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • RRPWRWPRWPWRR
    • Sequence Length

    • 13
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.26046345] Gram-negative bacteria: Escherichia coli ATCC 25922(MIC=31.25μg/ml), Pseudomonas aeruginosa ATCC 15442(MIC=31.25μg/ml), Acinetobacter baumanii ATCC 15308(MIC>250μg/ml), citrobacter spp(MIC>250μg/ml), Klebsiella pneumoniae(MIC>250μg/ml) ;
      • Gram-positive bacteria: Streptococcus pneumoniae(30 clinical isolates)(MIC=62.5μg/ml), Staphylococcus aureus ATCC 25923(MIC=31.25μg/ml), Methicillin resistant Staphylococcus aureus (MRSA)(MIC=62.5μg/ml), Enterococcus cloacae(MIC>250μg/ml),
    • Hemolytic Activity

      • [Ref.26046345] The hemolytic activities of IN3 is 2.39% at 250μg/ml against human RBCs.
    • Cytotoxicity

      • [Ref.26046345] The cell viability of NL-20 cell line is not affected (<10%) below 62.5 μg/ml. The cell viability of NL-20 is 93% and 26% at peptide concentrations of 125 and 250 μg/ml.
      • The cell viability of WRL-68 cell line is not affected (<10%) bel
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation
    • Nonterminal Modifications and Unusual Amino Acids

    • None
    • Stereochemistry

    • L
    • Structure

    • Not found
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP20928 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP20928.
    • Formula

    • C95H135N35O14
    • Absent Amino Acids

    • ACDEFGHIKLMNQSTVY
    • Common Amino Acids

    • R
    • Mass

    • 1991.34
    • PI

    • 12.7
    • Basic Residues

    • 6
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 4
    • Net Charge

    • +6
    • Boman Index

    • -8020
    • Hydrophobicity

    • -2.723
    • Aliphatic Index

    • 0
    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 22000
    • Absorbance 280nm

    • 1833.33
    • Polar Residues

    • 0

DRAMP20928

    • Function

    • Exhibits antimicrobial activity against the Gram-positive and Gram-negative bacteria.
  • ·Literature 1
    • Title

    • Antimicrobial Activity of Novel Synthetic Peptides Derived from Indolicidin and Ranalexin against Streptococcus pneumoniae.
    • Reference

    • PLoS One. 2015 Jun 5;10(6):e0128532.
    • Author

    • Jindal HM, Le CF, Mohd Yusof MY, Velayuthan RD, Lee VS, Zain SM, Isa DM, Sekaran SD.