General Information
-
DRAMP ID
- DRAMP20932
-
Peptide Name
- Hp1404
-
Source
- Heterometrus petersii
-
Family
- Melittin
-
Gene
- Not found
-
Sequence
- GILGKLWEGVKSIF
-
Sequence Length
- 14
-
UniProt Entry
- No entry found
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
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Target Organism
-
- [Ref.29379033] Gram-negative bacteria: Pseudomonas aeruginosa ATCC 27853 (MIC=12.5 μM), Pseudomonas aeruginosa 138 (MIC=12.5 μM), Pseudomonas aeruginosa 431 (MIC=12.5 μM), Pseudomonas aeruginosa 434 (MIC=12.5 μM), Pseudomonas aeruginosa 557 (MIC=6.25 μM), Pseudomonas aeruginosa 559 (MIC=25 μM), Pseudomonas aeruginosa 778 (MIC=12.5 μM), Pseudomonas aeruginosa 1034 (MIC=6.25 μM), Pseudomonas aeruginosa 1162 (MIC=12.5 μM), Pseudomonas aeruginosa 3290 (MIC=12.5 μM), Pseudomonas aeruginosa 3399 (MIC=6.25 μM), Pseudomonas aeruginosa 3543 (MIC=6.25 μM), Pseudomonas aeruginosa 3592 (MIC=6.25 μM), Pseudomonas aeruginosa 3904 (MIC=6.25 μM), Pseudomonas aeruginosa 4007 (MIC=25 μM), Pseudomonas aeruginosa 4319 (MIC=12.5 μM), Pseudomonas aeruginosa 4891 (MIC=6.25 μM), Pseudomonas aeruginosa 5018 (MIC=12.5 μM), Pseudomonas aeruginosa 671973 (MIC=3.13 μM)
-
Hemolytic Activity
-
- [Ref.29379033]Hemolysis 2% at 12.5 μM against mouse red blood cell, hemolysis 11% at 25.0 μM against mouse red blood cell, hemolysis 55% at 50.0 μM against mouse red blood cell, hemolysis 92% at 100.0 μM against mouse red blood cell, hemolysis 100% at 200.0 μM against mouse red blood cell
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Cytotoxicity
-
- [Ref.29379033] The cell survial against HaCaT cells is 85%, 26%, 5% and 6% at peptide concentrations of 25, 50, 100 and 200 μM
-
Binding Target
- Lipopolysaccharide (LPS)-binding
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- The peptide adopts an alpha-helical conformation in the membrane-mimicking environments and the presence of PE:PG and PC:CH liposomes, adopts a random coil conformation when mixed with PC:CH:SM liposomes.
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Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20932.
Physicochemical Information
-
Formula
- C75H119N17O18
Absent Amino Acids
- ACDHMNPQRTY
Common Amino Acids
- G
Mass
- 1546.87
PI
- 8.59
Basic Residues
- 2
Acidic Residues
- 1
Hydrophobic Residues
- 7
Net Charge
- +1
-
Boman Index
- 1054
Hydrophobicity
- 0.671
Aliphatic Index
- 132.14
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 423.08
Polar Residues
- 4
DRAMP20932
Comments Information
Function
- Antibacterial activity against Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa.
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Pubmed ID
- 29379033
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Reference
- Sci Rep. 2018 Jan 29;8(1):1763.
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Author
- Kim MK, Kang HK, Ko SJ, Hong MJ, Bang JK, Seo CH, Park Y.