General Information

    • DRAMP ID

    • DRAMP20933

    • Peptide Name

    • Stigmurin

    • Source

    • Tityus stigmurus

    • Family

    • Derived from scorpion venom peptide

    • Gene

    • Not found

    • Sequence

    • FFSLIPSLVGGLISAFK

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal, Antiparasitic, Antiproliferative

    • Target Organism

      • [Ref.29670004] Gram-positive bacteria: Staphylococcus aureus ATCC 29213 (MIC=9.38 μM), Staphylococcus epidermidis ATCC 122225 (MIC=9.38 μM);
      • yeasts: Candida albicans ATCC 90028 (MIC=37.5 μM);
      • Stigmurin inhibit 90% of the epimastigote forms of Trypanosoma cruzi growth at 25 ?M;
      • HeLa cell line: IC50=7.98 μM

    • Hemolytic Activity

      • [Ref.29670004]Hemolysis 0% at 1.17 μM, hemolysis 1% at 2.34 μM, hemolysis 1% at 4.69 μM, hemolysis 0% at 9.38 μM, hemolysis 1% at 18.75 μM, hemolysis 1% at 37.50 μM, hemolysis 3% at 75.00 μM against human red blood cell

    • Cytotoxicity

      • [Ref.29670004] The cell viability of HeLa cell lines is 56%, 66%, 66%, 81%, 78%, 66% and 30% at peptide concentrations of 2, 4, 8, 10, 15, 20 and 40 μM.
      • The cell viability of 3T3 cell lines is 81%, 63%, 51%, 29%, 27%, 19% and 15% at peptide concentra

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20933 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20933.

Physicochemical Information

    • Formula

    • C89H138N18O21

    • Absent Amino Acids

    • CDEHMNQRTWY

    • Common Amino Acids

    • FLS

    • Mass

    • 1796.18

    • PI

    • 8.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +1

    • Boman Index

    • 2552

    • Hydrophobicity

    • 1.535

    • Aliphatic Index

    • 137.65

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 5

DRAMP20933

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive bacteria. Antifungal activity against yeasts.Antiparasitic Activity against epimastigote forms of Trypanosoma cruzi. Antiproliferative Activity against HeLa cells.

Literature Information

  • ·Literature 1

    • Title

    • Analogs of the Scorpion Venom Peptide Stigmurin: Structural Assessment, Toxicity, and Increased Antimicrobial Activity.

    • Reference

    • Toxins (Basel). 2018 Apr 18;10(4). pii: E161.

    • Author

    • Parente AMS, Daniele-Silva A, Furtado AA, Melo MA, Lacerda AF, Queiroz M, Moreno C, Santos E, Rocha HAO, Barbosa EG, Carvalho E, Silva-Júnior AA, Silva MS, Fernandes-Pedrosa MF.