General Information
-
DRAMP ID
- DRAMP20935
-
Peptide Name
- Macropin 1(solitary bee, insects, animals)
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Source
- Macropis fulvipes (Solitary bee) (Megilla fulvipes)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- GFGMALKLLKKVL
-
Sequence Length
- 13
-
UniProt Entry
- C0HL98
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.24616110] Gram-negative bacteria: Escherichia coli(MIC=3.0 μM), Pseudomonas aeruginosa(MIC=35.0 μM);
- Gram-positive bacteria: Bacillus subtilis (MIC=1.3 μM), Staphylococcus aureus(MIC=3.7 μM);
- Fungus: Candida albicans (MIC=6.3 μM).
- [Ref.29185466]Gram-positive bacteria:Staphylococcus aureus ATCC 25923 (MIC=3.13 μM), Staphylococcus aureus ATCC 29213 (MIC=3.13 μM), Listeria monocytogenes KCTC 3710 (MIC=25 μM);
- Gram-negative bacteria: Pseudomonas aeruginosa ATCC 27853 (MIC=6.25 μM), Pseudomonas aeruginosa ATCC 15692 (MIC=6.25 μM), Escherichia coli ATCC 25922 (MIC=6.25 μM)
-
Hemolytic Activity
-
- [Ref.24616110] LC50=160 μM against human red blood cells.
- [Ref.29185466]5% hemolysis at 25 μM against red blood cells, 93% hemolysis at 50 μM against red blood cells
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Cytotoxicity
-
- [Ref.29185466] In HaCaT cells, Macropin produced cell survival rates of 82% and 41% at peptide concentrations of 25 and 50 μM.
- In Raw cells, Macropin produce cell survival rates of 41% and 0% at peptide concentrations of 25 and 50 μM.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix,random coil
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20935.
Physicochemical Information
-
Formula
- C68H120N16O14S
Absent Amino Acids
- CDEHINPQRSTWY
Common Amino Acids
- L
Mass
- 1417.86
PI
- 10.3
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +3
-
Boman Index
- 1609
Hydrophobicity
- 1.031
Aliphatic Index
- 150
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 2
DRAMP20935
Comments Information
[Ref.24616110] Antimicrobial peptide active against Gram-positive bacteria like B.subtilis, S.aureus and L.monocytogenes as well as against Gram-negative bacteria like E.coli and P.aeruginosa with minimum inhibitory concentrations (MIC) between 1.3 ?M and 35 ?M.Active against fungus C.albicans (MIC=6.3 ?M).Has little hemolytic activity . Adopts an alpha-helical structure in a membrane mimic environment. [Ref.29185466] Acts by disrupting membranes and bacterial cell wall structures.Binds to peptidoglycan and lipopolysaccharide (LPS)
Literature Information
- ·Literature 1
-
Title
- Structure-activity study of macropin, a novel antimicrobial peptide from the venom of solitary bee Macropis fulvipes (Hymenoptera: Melittidae).
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Pubmed ID
- 24616110
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Reference
- J Pept Sci. 2014 Jun;20(6):375-84. doi: 10.1002/psc.2625. Epub 2014 Mar 11.
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Author
- Monincová L, Veverka V, Slaninová J, Buděšínský M, Fučík V, Bednárová L, Straka J, Ceřovský V.
- ·Literature 2
-
Title
- Macropis fulvipes Venom component Macropin Exerts its Antibacterial and Anti-Biofilm Properties by Damaging the Plasma Membranes of Drug Resistant Bacteria.
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Pubmed ID
- 29185466
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Reference
- Sci Rep. 2017 Nov 29;7(1):16580. doi: 10.1038/s41598-017-16784-6.
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Author
- Ko SJ, Kim MK, Bang JK, Seo CH, Luchian T, Park Y.