General Information

    • DRAMP ID

    • DRAMP20938

    • Peptide Name

    • Cbf-14

    • Source

    • Synthetic construct

    • Family

    • Belongs to cathelicidin

    • Gene

    • Not found

    • Sequence

    • RLLRKFFRKLKKSV

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.28522372]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=64 μg/ml), Staphylococcus aureus (MIC=32 μg/ml);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=32 μg/ml), Escherichia coli (MIC=32 μg/ml), Pseudomonas aeruginosa ATCC 27853 (MIC=16 μg/ml), Pseudomonas aeruginosa (MIC=32 μg/ml);
      • Yeasts: Candida albicans ATCC 10231 (MIC=64 μg/ml), Candida albicans (MIC=64 μg/ml)

    • Hemolytic Activity

      • [Ref.28522372]2% hemolysis at 800 μg/ml against mice red blood cells

    • Cytotoxicity

      • The percentages of possible occurring conformations involved helix, beta, turn and random are also quantified in various solutions

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix,beta-sheet,beta-turn,random coil

    • Structure Description

    • The percentages of possible occurring conformations involved helix, beta, turn and random are also quantified in various solutions

    • Helical Wheel Diagram

    • DRAMP20938 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20938.

Physicochemical Information

    • Formula

    • C86H151N27O16

    • Absent Amino Acids

    • ACDEGHIMNPQTWY

    • Common Amino Acids

    • K

    • Mass

    • 1819.32

    • PI

    • 12.31

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +7

    • Boman Index

    • -4560

    • Hydrophobicity

    • -0.621

    • Aliphatic Index

    • 104.29

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP20938

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative bacteria and Gram-positive bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Effective antimicrobial activity of a peptide mutant Cbf-14-2 against penicillin-resistant bacteria based on its unnatural amino acids

    • Reference

    • Eur J Pharm Sci. 2017 Jul 15;105:169-177.

    • Author

    • Kang W, Liu H, Ma L, Wang M, Wei S, Sun P, Jiang M, Guo M, Zhou C, Dou J.