General Information
-
DRAMP ID
- DRAMP20940
-
Peptide Name
- Polybia-MP1S-Q12K
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Source
- Synthetic construct
-
Family
- Derived from Polybia-MP1
-
Gene
- Not found
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Sequence
- IDWKKLLDAAKKIL
-
Sequence Length
- 14
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- [Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC=0.8 μM), Staphylococcus aureus (MIC=1.2 μM), Staphylococcus epidermidis (MIC=50 μM)
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Hemolytic Activity
-
- [Ref.29075946]9.6% hemolysis at 12.5μM,13.9% hemolysis at 25μM against human red blood cells
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- The oct-4-enyl hydrocarbon staple between Leu6 and Ala10
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Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- The peptide adopts a conformational structure with 81.8% alpha helix in the aqueous solution.
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20940.
Physicochemical Information
-
Formula
- C79H135N19O19
Absent Amino Acids
- CEFGHMNPQRSTVY
Common Amino Acids
- K
Mass
- 1655.06
PI
- 9.53
Basic Residues
- 4
Acidic Residues
- 2
Hydrophobic Residues
- 8
Net Charge
- +2
-
Boman Index
- -909
Hydrophobicity
- 0.036
Aliphatic Index
- 153.57
Half Life
-
- Mammalian:20 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 423.08
Polar Residues
- 0
DRAMP20940
Comments Information
Function
- Antibacterial activity against the Gram-positive bacteria, position 6 and 10 are cross-linked residues connected by the oct-4-enyl hydrocarbon staple
Literature Information
- ·Literature 1
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Title
- Antimicrobial activity and stability of stapled helices of polybia-MP1
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Pubmed ID
- 29075946
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Reference
- Arch Pharm Res. 2017 Dec;40(12):1414-1422.
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Author
- Luong HX, Kim DH, Lee BJ, Kim YW