General Information
-
DRAMP ID
- DRAMP20944
-
Peptide Name
- [Pro3,DLeu9]TL(11) (Temporin L peptide derivative)
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Source
- Synthetic construct
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Family
- Derived from Temporin L
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Gene
- Not found
-
Sequence
- FVPWFSKFLWRIL
-
Sequence Length
- 13
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.28863356] Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=3.125μM), Staphylococcus aureus Cowan I (MIC=3.125μM), Staphylococcus epidermidis ATCC 12228 (MIC=1.56μM);
- Gram-negative bacteria: Acinetobacter baumannii ATCC 19606 (MIC=3.125μM), Escherichia coli D21 (MIC=25μM), Pseudomonas syringae pv tabaci 1918 (MIC=3.125μM), Pseudomonas aeruginosa ATCC 2785 (MIC=50μM);
- Yeasts: Candida albicans ATCC 10231 (MIC=3.125μM),Candida crusei ATCC 6258 (MIC=3.125μM)
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Hemolytic Activity
-
- [Ref.28863356]19.21 ± 1.44% cytotoxic effect at 12.5μM, 35.34 ± 5.99% cytotoxic effect at 25μM, 96.77 ± 0.81% cytotoxic effect at 50 μM on HaCaT cells.
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Cytotoxicity
-
- [Ref.28863356] The cytotoxic effect is 19.21±1.44%, 35.34±5.99% and 96.77±0.81% at 12.5, 25 and 50 μM
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- Mixed (D-Leu9, D-Trp10)
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Structure
- Alpha helix
-
Structure Description
- The analogues bearing a D residue showed a reduction in the percentage of helicity compared to the corresponding L analogues in the MMs
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20944.
Physicochemical Information
-
Formula
- C92H127N19O15
Absent Amino Acids
- ACDEGHMNQTY
Common Amino Acids
- F
Mass
- 1739.14
PI
- 11
Basic Residues
- 2
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +2
-
Boman Index
- 853
Hydrophobicity
- 0.931
Aliphatic Index
- 112.31
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 11000
Absorbance 280nm
- 916.67
Polar Residues
- 1
DRAMP20944
Comments Information
Function
- Antimicrobial activity against the Gram-negative bacteria, Gram-positive bacteria and Fungi. The position 9 of [Pro3,DLeu9]TL(10) is D-Leucine, D-Tryptophan replace L-Proline at the position 10 on the peptide [Pro3,DLeu9]TL(2).
Literature Information
- ·Literature 1
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Title
- Glycine-replaced derivatives of [Pro3,DLeu9]TL, a temporin L analogue: Evaluation of antimicrobial, cytotoxic and hemolytic activities
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Pubmed ID
- 28863356
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Reference
- Eur J Med Chem. 2017 Oct 20;139:750-761.
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Author
- Merlino F, Carotenuto A, Casciaro B, Martora F, Loffredo MR, Di Grazia A, Yousif AM, Brancaccio D, Palomba L, Novellino E, Galdiero M, Iovene MR, Mangoni ML, Grieco P.