• DRAMP ID

    • DRAMP20957
    • Peptide Name

    • StigA6
    • Source

    • Synthetic construct
    • Family

    • Derived from scorpion venom peptide
    • Gene

    • Not found
    • Sequence

    • FFSLIPKLVKGLISAFK
    • Sequence Length

    • 17
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiparasitic, Antiproliferative
    • Target Organism

      • [Ref.29670004] Gram-positive bacteria: Staphylococcus aureus ATCC 29213 (MIC=2.34 μM), Staphylococcus epidermidis ATCC 122225 (MIC=9.38 μM), Enterococcus faecalis ATCC 4028 (MIC=1.17 μM);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=4.69 μM), Enterobacter cloacae ATCC 13047 (MIC=18.75 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=9.38 μM);
      • yeasts: Candida albicans ATCC 90028 (MIC=9.38 μM), Candida krusei ATCC 6258 (MIC=37.5 μM), Candida glabrata ATCC 90030 (MIC=18.75 μM);
      • StigA6 inhibit 100% of the epimastigote forms of Trypanosoma cruzi growth at 2.5 µM;
      • HeLa cell line: IC50=14.01 µM
    • Hemolytic Activity

      • [Ref.29670004] Hemolysis 0% at 1.17 μM, hemolysis 2% at 2.34 μM, hemolysis 15% at 4.69 μM, hemolysis 27% at 9.38 μM, hemolysis 30% at 18.75 μM, hemolysis 30% at 37.50 μM, hemolysis 27% at 75.00 μM against human red blood cells
    • Cytotoxicity

      • [Ref.29670004] The cell viability of HeLa cell lines is 19%, 15%, 11%, 29%, 9%, 13% and 8% at peptide concentrations of 2, 4, 8, 10, 15, 20 and 40 μM.
      • The cell viability of 3T3 cell lines is 81%, 73%, 69%, 61%, 51%, 20% and 15% at peptide concentrati
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation
    • Nonterminal Modifications and Unusual Amino Acids

    • None
    • Stereochemistry

    • L
    • Structure

    • Alpha helix
    • Structure Description

    • The peptide adopts helical conformation with some random structure at the N- and C-terminals. In sodium phosphate buffer (PBS) and water they have a predominantly random structure, but in 20 mM sodium dodecyl sulfate (SDS) and 2,2,2-trifluoroethanol (TFE) they have a typical alpha-helix structure.
    • Helical Wheel Diagram

    • DRAMP20957 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP20957.
    • Formula

    • C96H154N20O20
    • Absent Amino Acids

    • CDEHMNQRTWY
    • Common Amino Acids

    • FKL
    • Mass

    • 1908.4
    • PI

    • 10.3
    • Basic Residues

    • 3
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 10
    • Net Charge

    • +3
    • Boman Index

    • 1688
    • Hydrophobicity

    • 1.147
    • Aliphatic Index

    • 137.65
    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 3

DRAMP20957

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria. Antifungal activity against yeasts.Antiparasitic Activity against epimastigote forms of Trypanosoma cruzi. Antiproliferative Activity against HeLa cells.
  • ·Literature 1
    • Title

    • Analogs of the Scorpion Venom Peptide Stigmurin: Structural Assessment, Toxicity, and Increased Antimicrobial Activity.
    • Reference

    • Toxins (Basel). 2018 Apr 18;10(4). pii: E161.
    • Author

    • Parente AMS, Daniele-Silva A, Furtado AA, Melo MA, Lacerda AF, Queiroz M, Moreno C, Santos E, Rocha HAO, Barbosa EG, Carvalho E, Silva-Júnior AA, Silva MS, Fernandes-Pedrosa MF.