General Information
-
DRAMP ID
- DRAMP20957
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Peptide Name
- StigA6
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Source
- Synthetic construct
-
Family
- Derived from scorpion venom peptide
-
Gene
- Not found
-
Sequence
- FFSLIPKLVKGLISAFK
-
Sequence Length
- 17
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiparasitic, Antiproliferative
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Target Organism
-
- [Ref.29670004] Gram-positive bacteria: Staphylococcus aureus ATCC 29213 (MIC=2.34 μM), Staphylococcus epidermidis ATCC 122225 (MIC=9.38 μM), Enterococcus faecalis ATCC 4028 (MIC=1.17 μM);
- Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=4.69 μM), Enterobacter cloacae ATCC 13047 (MIC=18.75 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=9.38 μM);
- yeasts: Candida albicans ATCC 90028 (MIC=9.38 μM), Candida krusei ATCC 6258 (MIC=37.5 μM), Candida glabrata ATCC 90030 (MIC=18.75 μM);
- StigA6 inhibit 100% of the epimastigote forms of Trypanosoma cruzi growth at 2.5 µM;
- HeLa cell line: IC50=14.01 µM
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Hemolytic Activity
-
- [Ref.29670004] Hemolysis 0% at 1.17 μM, hemolysis 2% at 2.34 μM, hemolysis 15% at 4.69 μM, hemolysis 27% at 9.38 μM, hemolysis 30% at 18.75 μM, hemolysis 30% at 37.50 μM, hemolysis 27% at 75.00 μM against human red blood cells
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Cytotoxicity
-
- [Ref.29670004] The cell viability of HeLa cell lines is 19%, 15%, 11%, 29%, 9%, 13% and 8% at peptide concentrations of 2, 4, 8, 10, 15, 20 and 40 μM.
- The cell viability of 3T3 cell lines is 81%, 73%, 69%, 61%, 51%, 20% and 15% at peptide concentrati
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- The peptide adopts helical conformation with some random structure at the N- and C-terminals. In sodium phosphate buffer (PBS) and water they have a predominantly random structure, but in 20 mM sodium dodecyl sulfate (SDS) and 2,2,2-trifluoroethanol (TFE) they have a typical alpha-helix structure.
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20957.
Physicochemical Information
-
Formula
- C96H154N20O20
Absent Amino Acids
- CDEHMNQRTWY
Common Amino Acids
- FKL
Mass
- 1908.4
PI
- 10.3
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 10
Net Charge
- +3
-
Boman Index
- 1688
Hydrophobicity
- 1.147
Aliphatic Index
- 137.65
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 3
DRAMP20957
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria. Antifungal activity against yeasts.Antiparasitic Activity against epimastigote forms of Trypanosoma cruzi. Antiproliferative Activity against HeLa cells.
Literature Information
- ·Literature 1
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Title
- Analogs of the Scorpion Venom Peptide Stigmurin: Structural Assessment, Toxicity, and Increased Antimicrobial Activity.
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Pubmed ID
- 29670004
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Reference
- Toxins (Basel). 2018 Apr 18;10(4). pii: E161.
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Author
- Parente AMS, Daniele-Silva A, Furtado AA, Melo MA, Lacerda AF, Queiroz M, Moreno C, Santos E, Rocha HAO, Barbosa EG, Carvalho E, Silva-Júnior AA, Silva MS, Fernandes-Pedrosa MF.