General Information
-
DRAMP ID
- DRAMP21084
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Peptide Name
- ccTP 6 (Derived from TP2)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- GWCRCVCRRGICRCFCRK
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Sequence Length
- 18
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.10673369] Gram-positive bacteria : Staphylococcus aureus(MIC=1.7 μM(low-salt);MIC=0.7 μM(high-salt)), Micrococcus luteus(MIC=0.3 μM(low-salt);MIC=0.4 μM(high-salt)), Enterococcus faecalis(MIC=1.4 μM(low-salt);MIC=1.2 μM(high-salt));
- Gram-negative bacteria : Escherichia coli(MIC=6.1 μM(low-salt);MIC=1.2 μM(high-salt)), Pseudomonas aeruginosa(MIC=2.0 μM(low-salt);MIC=4.7 μM(high-salt)), Primula vulgaris(MIC=17.0 μM(low-salt);MIC=39.8 μM(high-salt)), Klebsiella oxytoca(MIC=4.1 μM(low-salt);MIC=4.1 μM(high-salt));
- Fungi : Candida albicans(MIC=10.5 μM(low-salt);MIC=5.2 μM(high-salt)), Candida kefyr(MIC=1.1 μM(low-salt);MIC=0.7 μM(high-salt)), Candida tropicalis(MIC=2.3 μM(low-salt);MIC=1.9 μM(high-salt))
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Hemolytic Activity
-
- [Ref.10673369] EC25=3900 μM against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation (Gly18)
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys3 and Cys16, Cys5 and Cys14, Cys7 and Cys12.
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Stereochemistry
- L
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Structure
- Beta tile
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Structure Description
- Although there is insufficient information to deconvolute these CD spectra of these two-b-strand cyclic cystine-knot structures due to the various contributions by
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21084.
Physicochemical Information
-
Formula
- C89H149N35O19S6
Absent Amino Acids
- ADEHLMNPQSTY
Common Amino Acids
- C
Mass
- 2205.75
PI
- 9.57
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 4
Net Charge
- +6
-
Boman Index
- -5632
Hydrophobicity
- -0.089
Aliphatic Index
- 37.78
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5875
Absorbance 280nm
- 345.59
Polar Residues
- 8
DRAMP21084
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Candida kefyr, Candida tropicalis
Literature Information
- ·Literature 1
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Title
- Marked Increase in Membranolytic Selectivity of Novel Cyclic Tachyplesins Constrained with an Antiparallel Two-b Strand Cystine Knot Framework
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Pubmed ID
- 10673369
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Reference
- Biochem Biophys Res Commun. 2000 Jan 27;267(3):783-90.
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Author
- James P. Tam, Yi-An Lu, and Jin-Long Yang