General Information
-
DRAMP ID
- DRAMP21087
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Peptide Name
- PR-PG (Derived from PRW4)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- RFRRLRWKTRWRLKKICYCRRRFCVCV
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Sequence Length
- 27
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.25818457] Gram-positive bacteria : Staphylococcus aureus 25923(MIC=29.6 mg/L), Staphylococcus aureus 29213(MIC=7.4 mg/L), Staphylococcus epidermidis 12228(MIC=7.4 mg/L), Enterococcus faecalis 29212(MIC=3.7 mg/L), Bacillus subtilis 11060(MIC=7.4 mg/L);
- Gram-negative bacteria : Escherichia coli 078(MIC=14.8 mg/L), Escherichia coli K88(MIC=1.9 mg/L), Escherichia coli ER2566(MIC=3.7 mg/L), Escherichia coli 44102(MIC=1.9 mg/L), Escherichia coli 1515(MIC=14.8 mg/L), Escherichia coli 25922(MIC=3.7 mg/L), Escherichia coli UB1005(MIC=7.4 mg/L), Salmonella typhimurium 7731(MIC=14.8 mg/L), Salmonella typhimurium 1402(MIC=7.4 mg/L), Pseudomonas aeruginosa 21625(MIC=14.8 mg/L), Pseudomonas aeruginosa 21630(MIC=7.4 mg/L), Pseudomonas aeruginosa 10419(MIC=14.8 mg/L), Pseudomonas aeruginosa 27853(MIC=14.8 mg/L), Salmonella pullorum 7913(MIC=14.8 mg/L)
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Hemolytic Activity
-
- [Ref.25818457] 0.5% hemolysis at 1.1 mg/L , 2% hemolysis at 2.3 mg/L , 10% hemolysis at 4.6 mg/L , 23% hemolysis at 9.2 mg/L , 30% hemolysis at 18.4 mg/L , 45% hemolysis at 36.8mg/L , 46% hemolysis at 73.6 mg/L , 47% hemolysis at 147.1 mg/L , 50% hemolysis at 294.3 mg/L against human red blood cells
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Cytotoxicity
-
- [Ref.25818457] The cell viability of PR-FG is 92.7%, 76%, 64%, 58%, 53%, 43%, 39%, 37% and 34% at peptide concentrations of 1.1, 2.3, 4.6, 9.2, 18.4, 36.8, 73.6, 147.1 and 294.3 μg/ml.
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
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Structure
- Random coil, alpha helix, beta Sheet
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21087.
Physicochemical Information
-
Formula
- C165H271N59O30S4
Absent Amino Acids
- ADEGHMNPQS
Common Amino Acids
- R
Mass
- 3689.58
PI
- 11.72
Basic Residues
- 12
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +12
-
Boman Index
- -11506
Hydrophobicity
- -0.737
Aliphatic Index
- 64.81
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 12740
Absorbance 280nm
- 490
Polar Residues
- 6
DRAMP21087
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
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Title
- Characterization of cell selectivity, physiological stability and endotoxin neutralization capabilities of a-helix-based peptide amphiphiles
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Pubmed ID
- 25818457
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Reference
- Biomaterials. 2015 Jun;52:517-30. doi: 10.1016/j.biomaterials.2015.02.063.
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Author
- Zhi Ma, Dandan Wei, Ping Yan, Xin Zhu, Anshan Shan , Zhongpeng Bi