General Information
-
DRAMP ID
- DRAMP21089
-
Peptide Name
- C4 (Derived from PRW4)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- RFRRLRCKTRCRLKKI
-
Sequence Length
- 16
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.25735802] Gram-positive bacteria : Staphylococcus aureus 29213(MIC>128 μM), Staphylococcus epidermidis 12228(MIC>128 μM), Enterococcus faecalis 29212(MIC>128 μM);
- Gram-negative bacteria : Escherichia coli 25922(MIC=2 μM), Escherichia coli 1005(MIC>128 μM), Salmonella typhimurium 7731(MIC128 μM)
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Hemolytic Activity
-
- [Ref.25735802] MHC5>256 μM against human red blood cells
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Cytotoxicity
-
- [Ref.25735802] The peptide exhibited no cytoxicity against HaCaT cells in the range of 0/25-256 μM.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
-
Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21089.
Physicochemical Information
-
Formula
- C91H169N37O18S2
Absent Amino Acids
- ADEGHMNPQSVWY
Common Amino Acids
- R
Mass
- 2133.7
PI
- 12.01
Basic Residues
- 9
Acidic Residues
- 0
Hydrophobic Residues
- 4
Net Charge
- +9
-
Boman Index
- -8844
Hydrophobicity
- -1.219
Aliphatic Index
- 73.13
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 8.33
Polar Residues
- 3
DRAMP21089
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Characterization of antimicrobial activity and mechanisms of low amphipathic peptides with different α-helical propensity
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Pubmed ID
- 25735802
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Reference
- Acta Biomater. 2015 May;18:155-67. doi: 10.1016/j.actbio.2015.02.023.
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Author
- Xin Zhu, Licong Zhang, Jue Wang, Zhi Ma, Wei Xu, Jianping Li, Anshan Shan