General Information
-
DRAMP ID
- DRAMP21136
-
Peptide Name
- P8 (Derived from P5)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- HRWWRWWRH
-
Sequence Length
- 9
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.28372989] Gram-positive bacteria : Bacillus subtilis(MIC=1 μM, MBC=1 μM), Staphylococcus aureus(MIC=16 μM, MBC=32 μM);
- Gram-negative bacteria : Escherichia coli(MIC=8 μM, MBC=32 μM)
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Hemolytic Activity
-
- [Ref.28372989] MHC5=86.67 μM against human red blood cells
-
Cytotoxicity
-
- [Ref.28372989] IC50 > 86.67 μM for hPBMCs. The cell viability of hPBMCs is 96%, 98.4%, 86.8%, 84.8% and 88.8% and at peptide concentrations of 0.33, 0.67, 6.67, 12.33 and 86.67 μM.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21136.
Physicochemical Information
-
Formula
- C74H92N26O10
Absent Amino Acids
- ACDEFGIKLMNPQSTVY
Common Amino Acids
- W
Mass
- 1505.71
PI
- 12.3
Basic Residues
- 5
Acidic Residues
- 0
Hydrophobic Residues
- 4
Net Charge
- +5
-
Boman Index
- -4476
Hydrophobicity
- -2.611
Aliphatic Index
- 0
Half Life
-
- Mammalian:3.5 hour
- Yeast:10 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 22000
Absorbance 280nm
- 2750
Polar Residues
- 0
DRAMP21136
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Modulating short tryptophan- and arginine-rich peptides activity by substitution with histidine.
-
Pubmed ID
- 28372989
-
Reference
- Biochim Biophys Acta Gen Subj. 2017 Jul;1861(7):1844-1854. doi: 10.1016/j.bbagen.2017.03.024.
-
Author
- Bacalum M, Janosi L, Zorila F, Tepes AM, Ionescu C, Bogdan E, Hadade N, Craciun L, Grosu I, Turcu I, Radu M