• • DRAMP ID

  • DRAMP21140

• • Peptide Name

  • AMP72 (De novo synthesis)

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • KGCALVKVRGLTLKVCK

• • Sequence Length

  • 17

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.23893489] Gram-positive bacteria : Staphylococcus aureus(MBC=6.88 μg/ml, 3.78 μM), MRSA(MBC=1.72 μg/ml, 0.94 μM), Streptococcus agalactiae(MBC>55 μg/ml, 100 μM);
    • Gram-negative bacteria : Pseudomonas aeruginosa(MBC=3.30 μg/ml, 1.81 μM), Escherichia coli(MBC=3.40 μg/ml, 1.87 μM), Salmonella enteritidis(MBC=3.20 μg/ml, 1.76 μM), Stenotrophomonas maltophilia(MBC=18.5 μg/ml, 10.19 μM)

• • Hemolytic Activity

  • • [Ref.23893489] <1% hemolysis at 1 μg/ml , <1% hemolysis at 12.5 μg/ml , 1.5% hemolysis at 100 μg/ml , 4.2% hemolysis at 250 μg/ml , 12.0% hemolysis at 500 μg/ml against sheep red blood cells

• • Cytotoxicity

  • • [Ref.23893489] The cytotoxicity (vs control) of P72 is 3% against HUVEC (endothelial) cells at a peptide concentration of 100 μg/ml.
    • The cytotoxicity (vs control) of P72 is 4% agaisnt WI-38 (fibroblast) cells at a peptide concentration of 100 μg/ml. #

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Random coil, alpha helix, beta sheet, beta turn

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP21140.

DRAMP21140

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

• ·Literature 1

• • Title

  • In vitro activity of novel in silico-developed antimicrobial peptides against a panel of bacterial pathogens.

• • Pubmed ID

  • 23893489

• • Reference

  • J Pept Sci. 2013 Sep;19(9):554-65. doi: 10.1002/psc.2532.

• • Author

  • Romani AA, Baroni MC, Taddei S, Ghidini F, Sansoni P, Cavirani S, Cabassi CS