General Information

    • DRAMP ID

    • DRAMP21141

    • Peptide Name

    • AMP126 (De novo synthesis)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KWCRKWQWRGVKFIKCV

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.23893489] Gram-positive bacteria : Staphylococcus aureus(MBC=46.5 μg/ml, 20.65 μM), MRSA(MBC>100 μg/ml, 55 μM), Streptococcus agalactiae(MBC=2.81 μg/ml, 1.24 μM);
      • Gram-negative bacteria : Pseudomonas aeruginosa(MBC=0.40 μg/ml, 0.17 μM), Escherichia coli(MBC=2.22 μg/ml, 0.98 μM), Salmonella enteritidis(MBC=10.8 μg/ml, 4.80 μM), Stenotrophomonas maltophilia(MBC=16.6 μg/ml, 7.37 μM)

    • Hemolytic Activity

      • [Ref.23893489] <1% hemolysis at 1 μg/ml , <1% hemolysis at 12.5 μg/ml , 5.30% hemolysis at 100 μg/ml , 6.9% hemolysis at 250 μg/ml , 12.5% hemolysis at 500 μg/ml against sheep red blood cells

    • Cytotoxicity

      • [Ref.23893489] The cytotoxicity (vs control) of P126 is 5% against HUVEC (endothelial) cells at a peptide concentration of 100 μg/ml.
      • The cytotoxicity (vs control) of P126 is 6% agaisnt WI-38 (fibroblast) cells at a peptide concentration of 100 μg/ml.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix, beta sheet, beta turn

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21141 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21141.

Physicochemical Information

    • Formula

    • C107H163N31O19S2

    • Absent Amino Acids

    • ADEHLMNPSTY

    • Common Amino Acids

    • K

    • Mass

    • 2251.79

    • PI

    • 10.48

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +6

    • Boman Index

    • -3111

    • Hydrophobicity

    • -0.618

    • Aliphatic Index

    • 57.06

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 16625

    • Absorbance 280nm

    • 1039.06

    • Polar Residues

    • 3

DRAMP21141

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • In vitro activity of novel in silico-developed antimicrobial peptides against a panel of bacterial pathogens.

    • Reference

    • J Pept Sci. 2013 Sep;19(9):554-65. doi: 10.1002/psc.2532.

    • Author

    • Romani AA, Baroni MC, Taddei S, Ghidini F, Sansoni P, Cavirani S, Cabassi CS