General Information

    • DRAMP ID

    • DRAMP21142

    • Peptide Name

    • AMP2041 (De novo synthesis)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • HKCAKIKWRGVHVKYCA

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.23893489] Gram-positive bacteria : Staphylococcus aureus(MBC=3.71 μg/ml, 1.83 μM), MRSA(MBC=2.25 μg/ml, 1.11 μM), Streptococcus agalactiae(MBC>100 μg/ml, 55 μM);
      • Gram-negative bacteria : Pseudomonas aeruginosa(MBC=4.35 μg/ml, 2.14 μM), Escherichia coli(MBC=1.79 μg/ml, 0.88 μM), Salmonella enteritidis(MBC=10.41 μg/ml, 5.13 μM), Stenotrophomonas maltophilia(MBC=7.00 μg/ml, 3.45 μM)

    • Hemolytic Activity

      • [Ref.23893489] <1% hemolysis at 1 μg/ml , <1% hemolysis at 12.5 μg/ml , 5.73% hemolysis at 100 μg/ml , 6.4% hemolysis at 250 μg/ml , 9.4% hemolysis at 500 μg/ml against sheep red blood cells

    • Cytotoxicity

      • [Ref.23893489] The cytotoxicity (vs control) of P2041 is 6% against HUVEC (endothelial) cells at a peptide concentration of 100 μg/ml.
      • The cytotoxicity (vs control) of P2041 is 3% agaisnt WI-38 (fibroblast) cells at a peptide concentration of 100 μg/m

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix, beta sheet, beta turn

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21142 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21142.

Physicochemical Information

    • Formula

    • C92H147N29O19S2

    • Absent Amino Acids

    • DEFLMNPQST

    • Common Amino Acids

    • K

    • Mass

    • 2027.48

    • PI

    • 9.9

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +7

    • Boman Index

    • -2413

    • Hydrophobicity

    • -0.447

    • Aliphatic Index

    • 68.82

    • Half Life

      • Mammalian:3.5 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 7115

    • Absorbance 280nm

    • 444.69

    • Polar Residues

    • 4

DRAMP21142

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • In vitro activity of novel in silico-developed antimicrobial peptides against a panel of bacterial pathogens.

    • Reference

    • J Pept Sci. 2013 Sep;19(9):554-65. doi: 10.1002/psc.2532.

    • Author

    • Romani AA, Baroni MC, Taddei S, Ghidini F, Sansoni P, Cavirani S, Cabassi CS