General Information

    • DRAMP ID

    • DRAMP21148

    • Peptide Name

    • KR-12-a3 (Derived from KR-12)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KRIVKRIKKWLR

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.24105706] Gram-positive bacteria : Bacillus subtilis (KCTC 3068)(MIC=2 μM), Staphylococcus epidermidis (KCTC 1917)(MIC=1 μM), Staphylococcus aureus (KCTC 1621)(MIC=2 μM);
      • Gram-negative bacteria : Escherichia coli (KCTC 1682)(MIC=2 μM), Pseudomonas aeruginosa (KCTC 1637)(MIC=8 μM), Salmonella typhimurium (KCTC 1926)(MIC=2 μM)

    • Hemolytic Activity

      • [Ref.24105706] HC50>800 μM against human red blood cells

    • Cytotoxicity

      • [Ref.24105706] The cell viability of RAW264.7 macrophage cells is 104.7%, 111.6%, 105.3%, 108.4%, 111.2%, 104.4%, 108.8% and 95.0% at peptide concentrations of 0.78, 1.56, 3.12, 6.25, 12.5, 25, 50 and 100 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix

    • Structure Description

    • The CD spectra for all the peptides in 10 mM sodium phosphate buffer (pH 7.2) displayed a negative band at approximately 200 nm, indicating that the structure is random. In presence of 0.1% LPS, all the peptides showed characteristic α-helical CD spectra with two dichroic minimal values at 208 and 222 nm and a positive band near 192 nm .

    • Helical Wheel Diagram

    • DRAMP21148 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21148.

Physicochemical Information

    • Formula

    • C76H138N26O13

    • Absent Amino Acids

    • ACDEFGHMNPQSTY

    • Common Amino Acids

    • K

    • Mass

    • 1624.1

    • PI

    • 12.31

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +7

    • Boman Index

    • -4583

    • Hydrophobicity

    • -1.083

    • Aliphatic Index

    • 121.67

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 500

    • Polar Residues

    • 0

DRAMP21148

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Short KR-12 analogs designed from human cathelicidin LL-37 possessing both antimicrobial and antiendotoxic activities without mammalian cell toxicity.

    • Reference

    • J Pept Sci. 2013 Nov;19(11):700-7. doi: 10.1002/psc.2552.

    • Author

    • Jacob B, Park IS, Bang JK, Shin SY