• DRAMP ID

    • DRAMP21151
    • Peptide Name

    • KR-12-a6 (Derived from KR-12)
    • Source

    • Synthetic construct
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • LRIVKLILKWLR
    • Sequence Length

    • 12
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic form
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.24105706] Gram-positive bacteria : Bacillus subtilis (KCTC 3068)(MIC=8 μM), Staphylococcus epidermidis (KCTC 1917)(MIC=2 μM), Staphylococcus aureus (KCTC 1621)(MIC=4 μM);
      • Gram-negative bacteria : Escherichia coli (KCTC 1682)(MIC=16 μM), Pseudomonas aeruginosa (KCTC 1637)(MIC=16 μM), Salmonella typhimurium (KCTC 1926)(MIC=4 μM)
    • Hemolytic Activity

      • [Ref.24105706] HC50=22 μM against human red blood cells
    • Cytotoxicity

      • [Ref.24105706] The cell viability of RAW264.7 macrophage cells is 100%, 99.1%, 100%, 98.8%, 99.9%, 85.3%, 5.9% and 0% at peptide concentrations of 0.78, 1.56, 3.12, 6.25, 12.5, 25, 50 and 100 μM.
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation
    • Nonterminal Modifications and Unusual Amino Acids

    • None
    • Stereochemistry

    • L
    • Structure

    • Random coil, alpha helix
    • Structure Description

    • The CD spectra for all the peptides in 10 mM sodium phosphate buffer (pH 7.2) displayed a negative band at approximately 200 nm, indicating that the structure is random. In presence of 0.1% LPS, all the peptides showed characteristic α-helical CD spectra with two dichroic minimal values at 208 and 222 nm and a positive band near 192 nm .
    • Helical Wheel Diagram

    • DRAMP21151 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP21151.
    • Formula

    • C76H135N21O13
    • Absent Amino Acids

    • ACDEFGHMNPQSTY
    • Common Amino Acids

    • L
    • Mass

    • 1551.04
    • PI

    • 12.02
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 8
    • Net Charge

    • +4
    • Boman Index

    • -505
    • Hydrophobicity

    • 0.892
    • Aliphatic Index

    • 219.17
    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 5500
    • Absorbance 280nm

    • 500
    • Polar Residues

    • 0

DRAMP21151

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
  • ·Literature 1
    • Title

    • Short KR-12 analogs designed from human cathelicidin LL-37 possessing both antimicrobial and antiendotoxic activities without mammalian cell toxicity.
    • Reference

    • J Pept Sci. 2013 Nov;19(11):700-7. doi: 10.1002/psc.2552.
    • Author

    • Jacob B, Park IS, Bang JK, Shin SY