General Information
-
DRAMP ID
- DRAMP21151
-
Peptide Name
- KR-12-a6 (Derived from KR-12)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- LRIVKLILKWLR
-
Sequence Length
- 12
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.24105706] Gram-positive bacteria : Bacillus subtilis (KCTC 3068)(MIC=8 μM), Staphylococcus epidermidis (KCTC 1917)(MIC=2 μM), Staphylococcus aureus (KCTC 1621)(MIC=4 μM);
- Gram-negative bacteria : Escherichia coli (KCTC 1682)(MIC=16 μM), Pseudomonas aeruginosa (KCTC 1637)(MIC=16 μM), Salmonella typhimurium (KCTC 1926)(MIC=4 μM)
-
Hemolytic Activity
-
- [Ref.24105706] HC50=22 μM against human red blood cells
-
Cytotoxicity
-
- [Ref.24105706] The cell viability of RAW264.7 macrophage cells is 100%, 99.1%, 100%, 98.8%, 99.9%, 85.3%, 5.9% and 0% at peptide concentrations of 0.78, 1.56, 3.12, 6.25, 12.5, 25, 50 and 100 μM.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Random coil, alpha helix
-
Structure Description
- The CD spectra for all the peptides in 10 mM sodium phosphate buffer (pH 7.2) displayed a negative band at approximately 200 nm, indicating that the structure is random. In presence of 0.1% LPS, all the peptides showed characteristic α-helical CD spectra with two dichroic minimal values at 208 and 222 nm and a positive band near 192 nm .
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21151.
Physicochemical Information
-
Formula
- C76H135N21O13
Absent Amino Acids
- ACDEFGHMNPQSTY
Common Amino Acids
- L
Mass
- 1551.04
PI
- 12.02
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 8
Net Charge
- +4
-
Boman Index
- -505
Hydrophobicity
- 0.892
Aliphatic Index
- 219.17
Half Life
-
- Mammalian:5.5 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 500
Polar Residues
- 0
DRAMP21151
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Short KR-12 analogs designed from human cathelicidin LL-37 possessing both antimicrobial and antiendotoxic activities without mammalian cell toxicity.
-
Pubmed ID
- 24105706
-
Reference
- J Pept Sci. 2013 Nov;19(11):700-7. doi: 10.1002/psc.2552.
-
Author
- Jacob B, Park IS, Bang JK, Shin SY