General Information

    • DRAMP ID

    • DRAMP21223

    • Peptide Name

    • IsCT-p (Derived from IsCT-P)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ILKKIWKpIKKLF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.16040002] Gram-positive bacteria : Staphylococcus aureus(KCTC 1621)(MIC=1 μM), MRSA (CCARM 3001)(MIC=2 μM), MRSA (CCARM 3543)(MIC=1 μM), Bacillus subtilis(KCTC 3068)(MIC=2 μM), Staphylococcus epidermidis(KCTC 1917)(MIC=1 μM);
      • Gram-negative bacteria : Escherichia coli(KCTC 1682)(MIC=2 μM), Pseudomonas aeruginosa(KCTC 1637)(MIC=2 μM), MDRPA (CCARM 2095)(MIC=2 μM), Salmonella typhimurium(KCTC 1926)(MIC=2 μM)

    • Hemolytic Activity

      • [Ref.16040002] 0% hemolysis at 200 μM against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • Mixed(D-Pro8)

    • Structure

    • Random coil

    • Structure Description

    • This result indicated that D-Pro in the central position of a short a-helical peptide provides more remarkable structural flexibility than L-Pro.

    • Helical Wheel Diagram

    • DRAMP21223 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21223.

Physicochemical Information

    • Formula

    • C80H134N18O12

    • Absent Amino Acids

    • ACDEGHMNPQRSTVY

    • Common Amino Acids

    • K

    • Mass

    • 1655.19

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +5

    • Boman Index

    • 216

    • Hydrophobicity

    • 0.269

    • Aliphatic Index

    • 150

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 0

DRAMP21223

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • The role of the central L- or D-Pro residue on structure and mode of action of a cell-selective alpha-helical IsCT-derived antimicrobial peptide.

    • Reference

    • Biochem Biophys Res Commun. 2005 Sep 9;334(4):1329-35.

    • Author

    • Lim SS, Kim Y, Park Y, Kim JI, Park IS, Hahm KS, Shin SY