General Information

    • DRAMP ID

    • DRAMP21228

    • Peptide Name

    • IsCT-a (Derived from IsCT-P)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ILKKIWKaIKKLF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.16871429] Gram-positive bacteria : Staphylococcus aureus (KCTC 1621)(MIC=1 μM), Bacillus subtilis (KCTC 3068)(MIC=2 μM), Staphylococcus epidermidis (KCTC 1917)(MIC=1 μM), MRSA (CCARM 3001)(MIC=2 μM), MRSA (CCARM 3543)(MIC=1 μM);
      • Gram-negative bacteria : Escherichia coli (KCTC 1682)(MIC=2 μM), Pseudomonas aeruginosa (KCTC 1637)(MIC=2 μM), Salmonella typhimurium (KCTC 1926)(MIC=2 μM), MDRPA (CCARM 2095)(MIC=2 μM)

    • Hemolytic Activity

      • [Ref.16871429] HC50>400 μM against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • The 'a' in sequence is Ala peptoid residue (N-methylglycine)

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21228 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21228.

Physicochemical Information

    • Formula

    • C80H134N18O12

    • Absent Amino Acids

    • ACDEGHMNPQRSTVY

    • Common Amino Acids

    • K

    • Mass

    • 1629.15

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +5

    • Boman Index

    • 216

    • Hydrophobicity

    • 0.269

    • Aliphatic Index

    • 150

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 0

DRAMP21228

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Mechanism of antibacterial action of a synthetic peptide with an Ala-peptoid residue based on the scorpion-derived antimicrobial peptide IsCT.

    • Reference

    • Biotechnol Lett. 2006 Sep;28(18):1431-7. 

    • Author

    • Lim SS, Yoon SP, Park Y, Zhu WL, Park IS, Hahm KS, Shin SY