General Information
-
DRAMP ID
- DRAMP21231
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Peptide Name
- S-6K-F17-3GN (Derived from S-6K-F17)
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Source
- Synthetic construct
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Family
- Not found
-
Gene
- Not found
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Sequence
- KKKKKKNGFAAWGAFGA
-
Sequence Length
- 17
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
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Target Organism
-
- [Ref.29275987] Gram-negative bacteria : Escherichia coli(MIC=4.2 μM)
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Hemolytic Activity
-
- [Ref.29275987] MHC2=587 μM against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation (Ala17)
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Nonterminal Modifications and Unusual Amino Acids
- The 'A' at position 10 and 14 are 2-(4'-pentenyl)alanine residues and there is a hydrocarbon staple crosslinking them.
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Stereochemistry
- L
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Structure
- Not found
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Structure Description
- Substitutions with polar, known ‘helix-breaker’ Gly residues led to losses in helical character until finally the peptide containing 3 Gly and 1 Asn (S- 6K-F17-3GN) shows a severe loss in helical structure.
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21231.
Physicochemical Information
-
Formula
- C87H137N25O19
Absent Amino Acids
- CDEHILMPQRSTVY
Common Amino Acids
- K
Mass
- 1837.2
PI
- 10.7
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +6
-
Boman Index
- -2159
Hydrophobicity
- -0.953
Aliphatic Index
- 23.53
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 343.75
Polar Residues
- 4
DRAMP21231
Comments Information
Function
- Antibacterial activity against Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides.
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Pubmed ID
- 29275987
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Reference
- Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020.
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Author
- Stone TA, Cole GB, Nguyen HQ, Sharpe S, Deber CM