General Information

    • DRAMP ID

    • DRAMP21232

    • Peptide Name

    • Ranatuerin-2PLx (R2PLx; Frogs, Amphibians, Animals)

    • Source

    • Lithobates palustris (Pickerel frog) (Rana palustris)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

    • Gene

    • Not found

    • Sequence

    • GIMDTVKNAAKNLAGQLLDKLKCKITAC

    • Sequence Length

    • 28

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.30279210] Gram-positive bacteria : Staphylococcus aureus(NCTC 10788)(MIC=32 μM, MBC=128 μM), MRSA (ATCC 12493)(MIC=256 μM, MBC=512 μM), Enterococcus faecalis(NCTC 12697)(MIC=128 μM, MBC=512 μM);
      • Gram-negative bacteria : Escherichia coli(NCTC 10418)(MIC=32 μM, MBC=64 μM), Pseudomonas aeruginosa(ATCC 27853)(MIC>512 μM, MBC>512 μM);
      • Fungi : Candida albicans(NCTC 1467)(MIC=256 μM, MBC=512 μM)

    • Hemolytic Activity

      • [Ref.30279210] 0% hemolysis at 4 μM , 1% hemolysis at 8 μM , 5% hemolysis at 16 μM , 10% hemolysis at 32 μM , 13% hemolysis at 64 μM , 20% hemolysis at 128 μM , 30% hemolysis at 256 μM , 44% hemolysis at 512 μM against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix

    • Structure Description

    • The CD spectra of both peptides shows the pattern of random coil in aqueous solution and a typical α-helix CD spectra in 50% of TFE solution.

    • Helical Wheel Diagram

    • DRAMP21232 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21232.

Physicochemical Information

    • Formula

    • C127H226N36O38S3

    • Absent Amino Acids

    • EFHPRSWY

    • Common Amino Acids

    • K

    • Mass

    • 2961.59

    • PI

    • 9.24

    • Basic Residues

    • 5

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +3

    • Boman Index

    • -2156

    • Hydrophobicity

    • 0.118

    • Aliphatic Index

    • 108.21

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.63

    • Polar Residues

    • 8

DRAMP21232

DRAMP21232 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Antifungal activity against Candida albicans

Literature Information

  • ·Literature 1

    • Title

    • A novel antimicrobial peptide, Ranatuerin-2PLx, showing therapeutic potential in inhibiting proliferation of cancer cells.

    • Reference

    • Biosci Rep. 2018 Nov 9;38(6). pii: BSR20180710. doi: 10.1042/BSR20180710.

    • Author

    • Chen X, Zhang L, Ma C, Zhang Y, Xi X, Wang L, Zhou M, Burrows JF, Chen T