General Information

    • DRAMP ID

    • DRAMP21309

    • Peptide Name

    • TL-4 (Derived from Temporin-4Tl (TL))

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • FVKWWSKWLKKIL

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.26311041] Gram-positive bacteria : Bacillus subtilis (KCTC 3068)(MIC=4 μM), Staphylococcus epidermidis (KCTC 1917)(MIC=8 μM), Staphylococcus aureus (KCTC 1621)(MIC=2 μM);
      • Gram-negative bacteria : Escherichia coli (KCTC 1682)(MIC=8 μM), Pseudomonas aeruginosa (KCTC 1637)(MIC=16 μM), Salmonella typhimurium (KCTC 1926)(MIC=2 μM)

    • Hemolytic Activity

      • [Ref.26311041] HC50=15.9 μM against human red blood cells

    • Cytotoxicity

      • [Ref.26311041] The cell viability of RAW264.7 macrophages cells induced by TL-4 is 116.7%, 118.2% and 87.2% at peptide concentrations of 2.5, 5 and 10 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21309 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21309.

Physicochemical Information

    • Formula

    • C92H136N20O15

    • Absent Amino Acids

    • ACDEGHMNPQRTY

    • Common Amino Acids

    • K

    • Mass

    • 1762.22

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +4

    • Boman Index

    • 317

    • Hydrophobicity

    • 0

    • Aliphatic Index

    • 112.31

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 16500

    • Absorbance 280nm

    • 1375

    • Polar Residues

    • 1

DRAMP21309

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Enhancement of the anti-inflammatory activity of temporin-1Tl-derived antimicrobial peptides by tryptophan, arginine and lysine substitutions.

    • Reference

    • J Pept Sci. 2015 Oct;21(10):779-85. doi: 10.1002/psc.2807.

    • Author

    • Rajasekaran G, Kamalakannan R, Shin SY