General Information
-
DRAMP ID
- DRAMP21312
-
Peptide Name
- 2W-2 (Derived from PMAP-36)
-
Source
- synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- RRLRWKTRWRLK
-
Sequence Length
- 12
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.31276398] Gram-positive bacteria:Staphylococcus aureus 29213 (MIC=16 μM), Staphylococcus aureus 25923 (MIC=16 μM), Staphylococcus epidermidis 12228 (MIC=16 μM);
- Gram-negative bacteria: Escherichia coli 25922 (MIC=16 μM), Escherichia coli 1005 (MIC=4 μM), Staphylococcus typhi (MIC=32 μM)
-
Hemolytic Activity
-
- [Ref.31276398] MHC>128μM. The minimum hemolytic concentration (MHC) is measured as the lowest concentration of the peptides that caused 5% hemolysis of human red blood cells (hRBCs).
-
Cytotoxicity
-
- [Ref.31276398] The cell viability of the murine macrophage cell line RAW264.7 induced by 2W-2 is 94.8%, 92.1%, 95.5%, 98.3%, 94.5%, 95%, 89.5% and 84.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- α-helix in 50%TFE
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21312.
Physicochemical Information
-
Formula
- C80H135N31O14
Absent Amino Acids
- ACDEFGHIMNPQSVY
Common Amino Acids
- R
Mass
- 1755.15
PI
- 12.6
Basic Residues
- 7
Acidic Residues
- 0
Hydrophobic Residues
- 4
Net Charge
- +7
-
Boman Index
- -7377
Hydrophobicity
- -2.1
Aliphatic Index
- 65
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 11000
Absorbance 280nm
- 1000
Polar Residues
- 1
DRAMP21312
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Design of Trp-rich dodecapeptides with broad-spectrum antimicrobial potency and membrane-disruptive mechanism
-
Pubmed ID
- 31276398
-
Reference
- J Med Chem. 2019 Aug 8;62(15):6941-6957. doi: 10.1021/acs.jmedchem.9b00290.
-
Author
- Lyu Y, Chen T, Shang L, Yang Y, Li Z, Zhu J, Shan A.