General Information
-
DRAMP ID
- DRAMP21323
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Peptide Name
- RTI (Derived from PMAP-36)
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Source
- synthetic construct
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Family
- Not found
-
Gene
- Not found
-
Sequence
- WRIRWKTRWRIK
-
Sequence Length
- 12
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.31276398] Gram-positive bacteria:Staphylococcus aureus 29213 (MIC=2 μM), Staphylococcus aureus 25923 (MIC=2 μM), Staphylococcus epidermidis 12228 (MIC=2 μM), Staphylococcus aureus 11011 (MIC=2 μM);
- Gram-negative bacteria: Escherichia coli 25922 (MIC=4 μM), Escherichia coli 1005 (MIC=2 μM), Staphylococcus typhi (MIC=2 μM), Escherichia coli 20411 (MIC=2 μM), Acinetobacter baumannii AB1901 (MIC=2 μM), Acinetobacter baumannii AB1902 (MIC=2 μM), Pseudomonas aeruginosa 25349 (MIC=8 μM).
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Hemolytic Activity
-
- [Ref.31276398] MHC>128μM. The minimum hemolytic concentration (MHC) is measured as the lowest concentration of the peptides that caused 5% hemolysis of human red blood cells (hRBCs).
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Cytotoxicity
-
- [Ref.31276398] The cell viability of the murine macrophage cell line RAW264.7 induced by RTI is 103.1%, 97.4%, 103.8%, 106.4%, 96.4%, 104.8%, 95.7% and 96.4% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- α-helix in 50%TFE
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21323.
Physicochemical Information
-
Formula
- C85H133N29O14
Absent Amino Acids
- ACDEFGHLMNPQSVY
Common Amino Acids
- R
Mass
- 1785.18
PI
- 12.48
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 5
Net Charge
- +6
-
Boman Index
- -5652
Hydrophobicity
- -1.683
Aliphatic Index
- 65
Half Life
-
- Mammalian:2.8 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 16500
Absorbance 280nm
- 1500
Polar Residues
- 1
DRAMP21323
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.
Literature Information
- ·Literature 1
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Title
- Design of Trp-rich dodecapeptides with broad-spectrum antimicrobial potency and membrane-disruptive mechanism
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Pubmed ID
- 31276398
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Reference
- J Med Chem. 2019 Aug 8;62(15):6941-6957. doi: 10.1021/acs.jmedchem.9b00301.
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Author
- Lyu Y, Chen T, Shang L, Yang Y, Li Z, Zhu J, Shan A.