• DRAMP ID

    • DRAMP21323
    • Peptide Name

    • RTI (Derived from PMAP-36)
    • Source

    • synthetic construct
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • WRIRWKTRWRIK
    • Sequence Length

    • 12
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic form
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.31276398] Gram-positive bacteria:Staphylococcus aureus 29213 (MIC=2 μM), Staphylococcus aureus 25923 (MIC=2 μM), Staphylococcus epidermidis 12228 (MIC=2 μM), Staphylococcus aureus 11011 (MIC=2 μM);
      • Gram-negative bacteria: Escherichia coli 25922 (MIC=4 μM), Escherichia coli 1005 (MIC=2 μM), Staphylococcus typhi (MIC=2 μM), Escherichia coli 20411 (MIC=2 μM), Acinetobacter baumannii AB1901 (MIC=2 μM), Acinetobacter baumannii AB1902 (MIC=2 μM), Pseudomonas aeruginosa 25349 (MIC=8 μM).
    • Hemolytic Activity

      • [Ref.31276398] MHC>128μM. The minimum hemolytic concentration (MHC) is measured as the lowest concentration of the peptides that caused 5% hemolysis of human red blood cells (hRBCs).
    • Cytotoxicity

      • [Ref.31276398] The cell viability of the murine macrophage cell line RAW264.7 induced by RTI is 103.1%, 97.4%, 103.8%, 106.4%, 96.4%, 104.8%, 95.7% and 96.4% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation
    • Nonterminal Modifications and Unusual Amino Acids

    • None
    • Stereochemistry

    • L
    • Structure

    • α-helix in 50%TFE
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP21323 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP21323.
    • Formula

    • C85H133N29O14
    • Absent Amino Acids

    • ACDEFGHLMNPQSVY
    • Common Amino Acids

    • R
    • Mass

    • 1785.18
    • PI

    • 12.48
    • Basic Residues

    • 6
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 5
    • Net Charge

    • +6
    • Boman Index

    • -5652
    • Hydrophobicity

    • -1.683
    • Aliphatic Index

    • 65
    • Half Life

      • Mammalian:2.8 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 16500
    • Absorbance 280nm

    • 1500
    • Polar Residues

    • 1

DRAMP21323

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.
  • ·Literature 1
    • Title

    • Design of Trp-rich dodecapeptides with broad-spectrum antimicrobial potency and membrane-disruptive mechanism
    • Reference

    • J Med Chem. 2019 Aug 8;62(15):6941-6957. doi: 10.1021/acs.jmedchem.9b00301.
    • Author

    • Lyu Y, Chen T, Shang L, Yang Y, Li Z, Zhu J, Shan A.