General Information
-
DRAMP ID
- DRAMP21452
-
Peptide Name
- peptide 29 (Derived from B1)
-
Source
- synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KKKLAFA
-
Sequence Length
- 7
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.30842436] Gram-positive bacteria: MRSP C22963(MIC=4-8 µg/ml), MSSA ATCC 29213(MIC=16-32 µg/ml);
- Gram-negative bacteria: Pseudomonas aeruginosa(MIC=32 µg/ml)
-
Hemolytic Activity
-
- [Ref.30842436] EC10=9μM, 46% hemolysis at 150 µM against human red blood cells
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- Residue 5 and residue 7 are 2-naphthylmethylglycine. Residue 1, residue 2 and residue 3 are N-(4-aminobutyl)glycine
-
Stereochemistry
- L
-
Structure
- Unknown
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21452.
Physicochemical Information
-
Formula
- C39H68N10O8
Absent Amino Acids
- CDEGHIMNPQRSTVWY
Common Amino Acids
- K
Mass
- 805.03
PI
- 10.3
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 4
Net Charge
- +3
-
Boman Index
- -513
Hydrophobicity
- -0.214
Aliphatic Index
- 84.29
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 0
DRAMP21452
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.
Literature Information
- ·Literature 1
-
Title
- Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections
-
Pubmed ID
- 30842436
-
Reference
- Sci Rep. 2019; 9: 3679. Published online 2019 Mar 6. doi: 10.1038/s41598-019-39042-3
-
Author
- Ines Greco, Agnete Plahn Emborg, Bimal Jana, Natalia Molchanova, Alberto Oddo, Peter Damborg, Luca Guardabassi, Paul R. Hansen