General Information
-
DRAMP ID
- DRAMP21461
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Peptide Name
- PP (De Novo Synthesis)
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Source
- synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- IHKFWRPGRWFKHI
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Sequence Length
- 14
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.30538681] Gram-positive bacteria:Staphylococcus aureus ATCC 29213(MIC=16μM), Staphylococcus aureus ATCC 43300(MIC=16μM), Staphylococcus epidermidis ATCC 12228(MIC=16μM), Streptococci faecalis ATCC 29212(MIC=8μM), Bacillus subtilis CMCC 63501(MIC=8μM);
- Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=8μM), Escherichia coli UB 1005(MIC=4μM), bacteria pyocyaneum ATCC 27853(MIC=4μM), Salmonella typhimurium ATCC 7731(MIC=8μM), Salmonella Pulloru C79-13(MIC=4μM)
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Hemolytic Activity
-
- [Ref.30538681] MHC>128μM against human blood cells.The minimum hemolytic concentration (MHC) is defined as the peptide concentration resulting in 5% hemolysis.
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Cytotoxicity
-
- [Ref.30538681] The cell viability of intestinal epithelial cells (IPEC-J2) induced by PP is 67.7%, 69.1%, 70.5%, 65.4%, 61.3%, 65.0%, 70.5% and 68.7% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM.
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
-
Structure
- A combination of α-helical and β-hairpin
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Structure Description
- In PBS, the α-helix content of PQ is 12.35% and the β-strand content of that is 16.30%. In SDS, the α-helix content of PQ is 9.86% and the β-strand of that is 11.56%. In TFE, the α-helix content of PQ is 37.54% and the β-strand of that is 2.54%.
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21461.
Physicochemical Information
-
Formula
- C95H134N28O15
Absent Amino Acids
- ACDELMNQSTVY
Common Amino Acids
- FHIKRW
Mass
- 1908.29
PI
- 12.02
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 6
Net Charge
- +6
-
Boman Index
- -2886
Hydrophobicity
- -0.886
Aliphatic Index
- 55.71
Half Life
-
- Mammalian:20 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 11000
Absorbance 280nm
- 846.15
Polar Residues
- 1
DRAMP21461
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.
Literature Information
- ·Literature 1
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Title
- Short Symmetric-End Antimicrobial Peptides Centered on β-Turn Amino Acids Unit Improve Selectivity and Stability
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Pubmed ID
- 30538681
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Reference
- Front Microbiol. 2018; 9: 2832. Published online 2018 Nov 27. doi: 10.3389/fmicb.2018.02832
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Author
- Na Dong, Shuli Chou, Jiawei Li, Chenyu Xue, Xinran Li, Baojing Cheng, Anshan Shan, and Li Xu