• DRAMP ID

    • DRAMP21462
    • Peptide Name

    • GG (De Novo Synthesis)
    • Source

    • synthetic construct
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • IHKFWRGGRWFKHI
    • Sequence Length

    • 14
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic form
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.30538681] Gram-positive bacteria:Staphylococcus aureus ATCC 29213(MIC=16μM), Staphylococcus aureus ATCC 43300(MIC=32μM), Staphylococcus epidermidis ATCC 12228(MIC=32μM), Streptococci faecalis ATCC 29212(MIC=32μM), Bacillus subtilis CMCC 63501(MIC=16μM);
      • Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=8μM), Escherichia coli UB 1005(MIC=8μM), bacteria pyocyaneum ATCC 27853(MIC=16μM), Salmonella typhimurium ATCC 7731(MIC=64μM), Salmonella Pulloru C79-13(MIC=8μM)
    • Hemolytic Activity

      • [Ref.30538681] MHC>128μM against human blood cells.The minimum hemolytic concentration (MHC) is defined as the peptide concentration resulting in 5% hemolysis.
    • Cytotoxicity

      • [Ref.30538681] The cell viability of intestinal epithelial cells (IPEC-J2) induced by GG is 67.7%, 74.2%, 76.5%, 72.4%, 72.8%, 69.1%, 65.9% and 59.9% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM.
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation
    • Nonterminal Modifications and Unusual Amino Acids

    • None
    • Stereochemistry

    • L
    • Structure

    • A combination of α-helical and β-hairpin
    • Structure Description

    • In PBS, the α-helix content of PQ is 1.49% and the β-strand content of that is 32.19%. In SDS, the α-helix content of PQ is 11.99% and the β-strand of that is 9.42%. In TFE, the α-helix content of PQ is 18.98% and the β-strand of that is 11.53%.
    • Helical Wheel Diagram

    • DRAMP21462 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP21462.
    • Formula

    • C92H130N28O15
    • Absent Amino Acids

    • ACDELMNPQSTVY
    • Common Amino Acids

    • FGHIKRW
    • Mass

    • 1868.22
    • PI

    • 12.02
    • Basic Residues

    • 6
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 6
    • Net Charge

    • +6
    • Boman Index

    • -2792
    • Hydrophobicity

    • -0.8
    • Aliphatic Index

    • 55.71
    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 11000
    • Absorbance 280nm

    • 846.15
    • Polar Residues

    • 2

DRAMP21462

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.
  • ·Literature 1
    • Title

    • Short Symmetric-End Antimicrobial Peptides Centered on β-Turn Amino Acids Unit Improve Selectivity and Stability
    • Reference

    • Front Microbiol. 2018; 9: 2832. Published online 2018 Nov 27. doi: 10.3389/fmicb.2018.02832
    • Author

    • Na Dong, Shuli Chou, Jiawei Li, Chenyu Xue, Xinran Li, Baojing Cheng, Anshan Shan, and Li Xu