• DRAMP ID

    • DRAMP21606
    • Peptide Name

    • U2
    • Source

    • Synthetic construct
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • KXAKWXK
    • Sequence Length

    • 7
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic form
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-
    • Target Organism

      • Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC > 100 μM), Staphylococcus aureus ATCC 6538p (MIC > 100 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC > 100 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM).
    • Hemolytic Activity

      • 0.76%, 0.86%, 0.84%, 1.05%, 0.97%, 0.93%, 0.96% and 0.88% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Acetylation
    • C-terminal Modification

    • Amidation
    • Nonterminal Modifications and Unusual Amino Acids

    • ①The X (position: 2 and 6) in sequence are (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged.
    • Stereochemistry

    • L
    • Structure

    • α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)
    • Structure Description

    • As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.
    • Helical Wheel Diagram

    • DRAMP21606 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP21606.
    • Formula

    • C₄₆H₇₇N₁₁O₈
    • Absent Amino Acids

    • CDEFGHILMNPQRSTVY
    • Common Amino Acids

    • K
    • Mass

    • 912.17
    • PI

    • 10.3
    • Basic Residues

    • 3
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 2
    • Net Charge

    • +3
    • Boman Index

    • -1251
    • Hydrophobicity

    • -1.543
    • Aliphatic Index

    • 14.29
    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:3 min
    • Extinction Coefficient Cystines

    • 5500
    • Absorbance 280nm

    • 916.67
    • Polar Residues

    • 0

DRAMP21606

    • Function

    • Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 100 μM.
  • ·Literature 1
    • Title

    • De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides
    • Reference

    • B KOREAN CHEM SOC
    • Author

    • Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim