General Information
-
DRAMP ID
- DRAMP21606
-
Peptide Name
- U2
-
Source
- Synthetic construct
-
Family
- Not found
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Gene
- Not found
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Sequence
- KXAKWXK
-
Sequence Length
- 7
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
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Target Organism
-
- Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC > 100 μM), Staphylococcus aureus ATCC 6538p (MIC > 100 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);
- Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC > 100 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM).
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Hemolytic Activity
-
- 0.76%, 0.86%, 0.84%, 1.05%, 0.97%, 0.93%, 0.96% and 0.88% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Acetylation
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- ①The X (position: 2 and 6) in sequence are (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged.
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Stereochemistry
- L
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Structure
- α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)
-
Structure Description
- As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21606.
Physicochemical Information
-
Formula
- C₄₆H₇₇N₁₁O₈
Absent Amino Acids
- CDEFGHILMNPQRSTVY
Common Amino Acids
- K
Mass
- 912.17
PI
- 10.3
Basic Residues
- 3
Acidic Residues
- 0
Hydrophobic Residues
- 2
Net Charge
- +3
-
Boman Index
- -1251
Hydrophobicity
- -1.543
Aliphatic Index
- 14.29
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:3 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 916.67
Polar Residues
- 0
DRAMP21606
Comments Information
Function
- Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 100 μM.
Literature Information
- ·Literature 1
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Title
- De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides
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Pubmed ID
- Not found
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Reference
- B KOREAN CHEM SOC
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Author
- Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim