• DRAMP ID

    • DRAMP21616
    • Peptide Name

    • DRIM
    • Source

    • Synthetic construct
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • qqrkrkiwsilaplgttlvklvagig
    • Sequence Length

    • 26
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic form
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC = 32 μg/mL), Enterococcus faecalis (MIC = 32 μg/mL);
      • Gram-negative bacteria: Escherichia coli (MIC = 32 μg/mL), Pseudomonas aeruginosa (MIC = 64 μg/mL)
    • Hemolytic Activity

      • [Ref.28921993] 60.5%, 98.0%, 95.1%, 99.0%, 97.9%, 96.3%, 98.8% and 98.2% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml.
    • Cytotoxicity

      • [Ref.28921993] The toxicity of DRIM toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM.
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Linear
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation
    • Nonterminal Modifications and Unusual Amino Acids

    • None
    • Stereochemistry

    • D
    • Structure

    • ①Disordered conformation in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②50
    • Structure Description

    • Our CD data show that all four linear peptides were generally disordered in aqueous solution, as expected, but prominent effects were seen regarding the impact of the staple.
    • Helical Wheel Diagram

    • DRAMP21616 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • There is no predicted structure for DRAMP21616.
    • Formula

    • C₁₃₁H₂₂₈N₃₈O₃₂
    • Absent Amino Acids

    • CDEFHMNY
    • Common Amino Acids

    • L
    • Mass

    • 2847.49
    • PI

    • 12.02
    • Basic Residues

    • 5
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 12
    • Net Charge

    • +5
    • Boman Index

    • -1482
    • Hydrophobicity

    • 0.273
    • Aliphatic Index

    • 135
    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:10 hour
    • Extinction Coefficient Cystines

    • 5500
    • Absorbance 280nm

    • 220
    • Polar Residues

    • 6

DRAMP21616

DRAMP21616 chydropathy plot
    • Function

    • Antibacterial activity against Gram-positive and Gram-negative bacteria.
  • ·Literature 1
    • Title

    • Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties.
    • Reference

    • J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26.
    • Author

    • Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich