General Information

    • DRAMP ID

    • DRAMP21622

    • Peptide Name

    • MAP-1

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KLALKALKALKAALKLA

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC = 16 μg/mL), Enterococcus faecalis (MIC = 256 μg/mL);
      • Gram-negative bacteria: Escherichia coli (MIC = 16 μg/mL), Pseudomonas aeruginosa (MIC = 256 μg/mL)

    • Hemolytic Activity

      • [Ref.28921993] 1.6%, 0.8%, 0.6%, 0.4%, 0.7%, 1.8%, 0.4% and 0.1% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml.

    • Cytotoxicity

      • [Ref.28921993] The toxicity of MAP-1 toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • ①Disordered conformation in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②Ty

    • Structure Description

    • Our CD data show that all four linear peptides were generally disordered in aqueous solution, as expected, but prominent effects were seen regarding the impact of the staple.

    • Helical Wheel Diagram

    • DRAMP21622 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21622.

Physicochemical Information

    • Formula

    • C₈₄H₁₅₈N₂₂O₁₈

    • Absent Amino Acids

    • CDEFGHIMNPQRSTVWY

    • Common Amino Acids

    • AL

    • Mass

    • 1764.31

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +5

    • Boman Index

    • 1263

    • Hydrophobicity

    • 0.829

    • Aliphatic Index

    • 172.94

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:3 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 0

DRAMP21622

Comments Information

    • Function

    • Antibacterial activity against Gram-positive and Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties.

    • Reference

    • J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26.

    • Author

    • Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich