General Information
-
DRAMP ID
- DRAMP29082
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Peptide Name
- dCATH 12-4 (modified from dCATH 12)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- N/A
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Sequence
- LIKKIYRKWKRW
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Sequence Length
- 12
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-, Anti-Gram+
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Target Organism
-
- [Ref.32698035] Gram-positive bacteria: S. aureus KCTC 1621 (MIC = 4 μM), S. epidermidis KCTC 1917 (MIC = 4 μM), B. subtilis KCTC 3068 (MIC = 2 μM);
- Resistant Gram-positive bacteria: MRSA CCARM 3089 (MIC = 16 μM), MRSA CCARM 3090 (MIC = 8 μM), MRSA CCARM 3095 (MIC = 8 μM), VREF ATCC 51559(MIC = 8 μM);
- Gram-negative bacteria: E. coli KCTC 1682 (MIC = 8 μM), P. aeruginosa KCTC 1637(MIC = 2 μM), S. typhimurium KCTC 1926 (MIC = 2 μM);
- Resistant Gram-negative bacteria: MDRPAd CCARM 2095 (MIC = 8 μM), MDRPA CCARM 2109 (MIC = 8 μM);
- Yeast: C. albicans KCTC 7965 (MIC = 8 μM), C. albicans KCTC 7121 (MIC = 4 μM).
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Hemolytic Activity
-
- [Ref.32698035] HC10 > 256 μM. Note: HC10 is the peptide concentration that caused 10% hemolysis of sheep red blood cells (sRBCs).
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Cytotoxicity
-
- [Ref.32698035] At the concentration of 1.25, 2.5, 5, 10, 20, 40, 80 μM, the cell survival of dCATH 12-4 against human bone marrow SH-SY5Y cells is 102%, 102%, 100%, 100%, 100%, 91%, 83%.
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Binding Target
- Bacterial DNA
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
-
Structure
- Random coil in buffer; 41.28% α-helix content in 50% TFE; 36.63% α-helix content in 30 mM SDS; 42.87% α-helix content in 0.1% LPS
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Structure Description
- Among the 12-meric analog AMPs, Trp rich dCATH 12–4 and dCATH 12-5 displayed high helicity in all membrane-mimetic environment with highest α-helical conformation observed (42.8% and 40.4% respectively) in 0.1% LPS
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP29082.
Physicochemical Information
-
Formula
- C85H136N24O14
Absent Amino Acids
- ACDEFGHMNPQSTV
Common Amino Acids
- K
Mass
- 1718.17
PI
- 11.22
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 5
Net Charge
- +6
-
Boman Index
- -3276
Hydrophobicity
- -1.242
Aliphatic Index
- 97.5
Half Life
-
- Mammalian:5.5 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 12490
Absorbance 280nm
- 1135.45
Polar Residues
- 1
DRAMP29082
Comments Information
Comment
- dCATH 12-4 demonstrated synergistic actions with various conventional antibiotics against antibiotic resistant pathogens, thus indicating their ability as promising adjuncts to combination therapy.
Literature Information
- ·Literature 1
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Title
- Antimicrobial and anti-inflammatory activities of short dodecapeptides derived from duck cathelicidin: Plausible mechanism of bactericidal action and endotoxin neutralization
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Pubmed ID
- 32698035
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Reference
- Eur J Med Chem. 2020 Oct 15;204:112580. doi: 10.1016/j.ejmech.2020.112580. Epub 2020 Jul 16.
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Author
- Kumar SD, Shin SY